一氧化氮供体对成年大鼠脑缺血后海马神经元再生的影响

目的　研究NO是否参与脑缺血后海马神经元再生。方法　血管内栓线法阻塞大脑中动脉 1.5h后再灌注 ,制备局灶脑缺血再灌性模型。脑缺血后 1h海马内输入 10 μLNO供体硝普钠 (0 .1mmol·L- 1)和维生素C(0 .4mmol·L- 1)。于脑缺血后 12 0h ,5 溴 2′ 脱氧尿嘧啶核苷 (BrdU)掺入法测定海马齿状回的细胞扩增。于再灌注后 2 4h测定海马诱导型一氧化氮合酶 (iNOS)活性及NO水平。结果在再灌注后d 8缺血侧海马齿状回的BrdU阳性细胞数比对照侧多大约 5倍 ,硝普钠和维生素C海马内输入显著增加脑缺血诱发的海马齿状回神经细胞扩增。结论　NO对脑缺血诱发海马齿状回神经元再生有重要作用

Effect of nitric oxide donor on neurogenesis after transient focal cerebral ischemia in adult rat hippocampus

AIM To determine whether NO is involved in neurogenesis in dentate gyrus after cerebral ischemia. METHODS The middle cerebral artery in rats was occluded for 1.5 h by an intraluminal filament and then reperfused to prepare transient focal cerebral ischemia. Normal saline 10 μL containing 0.1 mmol•L^-1 sodium nitroprusside(SNP) and 0.4 mmol•L^-1 vitamin C (VC) was infused into ipsilateral hippocampus 1 h after cerebral ischemia. A bromodeoxyuridine (BrdU) labeling method was used to identify the postproliferation cells in dentate gyrus 120 h after ischemia. The measurement of inducible nitric oxide synthase activity and NO concentration in the hippocampus were performed at 24 h after reperfusion. RESULTS The density of BrdU-positive cells in the ipsilateral dentate gyrus increased about 5-fold 8 d after ischemia, compared with the contralateral one. SNP and VC infusion significantly increased ischemia-induced cells proliferation in the dentate gyrus. CONCLUSION NO takes place an important effect in ischemia-stimulated cell reproducing in the dentate gyrus of adult rats.