咪唑斯汀抑制多形核白细胞白三烯LTB4合成的实验研究

目的：研究咪唑斯汀的抗炎机制。方法：采用皮下植入0．5％花生四烯酸浸泡的海绵做为抗原刺激剂的方法。各组分别在植入前、后2h灌胃给药地塞米松（0．1mg／kg）、咪唑斯汀高剂量（0．3mg／kg）、咪唑斯汀低剂量（0．1mg／kg）、氯雷他定（0．3mg／kg），结合高效液相的分析方法，定量检测炎症关键产物——白三烯LTB4的生成量。结果：与模型对照组比较，地塞米松组、咪唑斯汀高剂量组有较强的抑制作用（P＜0．01）、咪唑斯汀低剂量组显示出抑制作用（P＜0．05）、而氯雷他定组无抑制作用（P＞0．01）。结论：咪唑斯汀可有效抑制多形核白细胞合成LTB4，咪唑斯汀的抗炎作用机制可能主要通过抑制5-脂氧合酶活性发挥作用。

Experimental study on inhibition of LTB4 production of mizolastine

Objective:To study the anti-inflammatory mechanism of mizolastine in rats.Methods:The sponge was soaked in normal saline with 0.5% arachidonic acid,and implanted in the subcutaneous tissue of rats mizolastine(0.3 mg/kg),dexamethasone(0.1 mg/kg) and loratadine(0.3 mg/kg) were given orally 2 hours before and after the implantation.The production of LTB4 was measured by using HPLC.Results:Mizolastine (0.3 mg/kg) and dexamethasone significantly inhibited the production of LTB4 induced by arachidonic acid,while loratadine had no significant inhibitory effects.Conclusions:The results suggest that mizolastine may exert its anti-inflammatory activity by inhibiting 5-lipogense.