抑癌基因KLF6在肝癌HepG2细胞修复DNA损伤过程中的作用研究

目的：研究抑癌基因KLF6对肝癌HepG2细胞修复DNA损伤能力的影响。 方法: RT-PCR及Western blotting技术测定HepG2细胞在5 mg/L顺铂作用12、24、36 h后KLF6 mRNA及其蛋白水平变化。采用宿主细胞再活化反应(HCR)检测KLF6稳定表达HepG2细胞修复DNA损伤的能力。结果： 在5 mg/L顺铂作用下，随作用时间延长，KLF6 mRNA及其蛋白水平增高，但作用36 h，KLF6 蛋白水平下降。KLF6稳定表达HepG2细胞修复被顺铂损伤质粒DNA的能力明显高于对照组。结论： DNA损伤能诱导肝癌HepG2细胞KLF6基因表达，KLF6基因在细胞DNA损伤修复过程中发挥一定作用。

Effect of KLF6 gene on nucleotide excision repair capacity in human hepatocellular carcinoma cell line HepG2

AIM: To investigate the roles of KLF6 gene on nucleotide excision repair capacity of human hepatocellular carcinoma cell line HepG2. METHODS: Expression of KLF6 was detected by RT-PCR and Western blotting, followed by HepG2 cells treated with cisplatin (5 mg/L). Host cell reactivation assay was used to assess nucleotide excision capacity of cisplatin-damaged HepG2 cells. RESULTS: Upregulation of KLF6 mRNA or protein in HepG2 cells treated with 5 mg/L concentration of cisplatin was in a time-dependent manner, but KLF6 protein was degraded when exposed to cisplatin at same dose (5 mg/L) for 36 h. HCR assay showed that NER capacity of KLF6 overexpressing HepG2 cells was significantly enhanced, compared to that in control cells. CONCLUSION: DNA damage induced by cisplatin induces the expression of KLF6 gene. KLF6 gene may play an important role in mechanism of nucleotide excision repair in HepG2 cells.