塑料条ELISA检测日本血吸虫病抗体方法的建立及应用

塑料条ELISA(PS-ELISA)方法,以PVC塑料条代替微孔板,作为ELISA的固相载体,以达到简化操作、节约成本的目的.建立的PS-ELISA方法,在抗原、血清、酶结合物工作浓度选择时,与常规ELISA方法相比,两法OD值差异不大;检测73份血吸虫病人血清、95份健康人血清、18份肺吸虫病人血清和18份丝虫病人血清,PS-ELISA和ELISA检测阳性率分别为95.9%、1%、0%、0%和94.5%、1%、0%、0%,两法无显著差异;检测73份血吸虫病人血清,PS-ELISA和ELISA的平均P/N值分别为14.63和5.47,t检验有显著差异(t=12.28,P<0.01);检测95份健康人血清,PS-ELISA和ELISA的平均P/N值分别为1.01和1.16,(t=1.92,P>0.05).结果提示,PS-ELISA可应用于检测血吸虫病,且该法似能提高低感染度的检出率和降低假阳性率.

DEVELOPMENT OF PLASTIC STRIP-ELISA AND APPLICATION FOR DETECTING ANTIBODIES OF SCHISTOSOMA JAPONICA

The plastic strip-ELISA (PS-ELISA) was developed,in which PVC plastic strip substituted for microtitre plate as the solid phase. The method was simpl and the cost less. Compared with routine ELISA, the concentrations of antigen ,serum and enzyme conjugate were similar between the two methods. Sera of 73 chronic patients of schistosomiasis, 95 health persons, 18 patients of paragonimus westermani and 18 patients of wachereria bancrofit were detected by PS-ELISA and ELISA. The positive rate were 95. 9%,1%,0%,0% respectively by PS-ELISA,and 94. 5% ,1% ,0% ,0% by ELISA. There is no siginificant difference the between two methods. The mean P/N values of 73 sera of schistosomiasis detected by PS-ELISA and ELISA were 14. 63 and 5. 47,and the mean P/N values of 95 sera of health persons detected were 1. 0 and 1. 16 respectively. It indicated that PS-ELISA may play a role in detecting lower infective density of schistosomiasis and reducing the false positive rate.