野山参与园参抗坏血酸-谷胱甘肽循环代谢差异的比较

目的通过比较野山参与园参在抗坏血酸-谷胱甘肽(AsA-GSH)循环中代谢相关酶活性及代谢物的量,探讨野山参与园参抗氧化能力差异。方法氮蓝四唑法测定超氧化物歧化酶(SOD)活性,高锰酸钾滴定法测定过氧化氢酶(CAT)活性,UV法测定抗氧化酶活性和抗氧化物的量,利用氨基酸自动分析仪检测谷氨酸、半胱氨酸、甘氨酸量。结果野山参中SOD、CAT活性高于园参,在AsA-GSH循环中野山参抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、谷胱甘肽还原酶(GR)等抗氧化酶活性均高于园参,谷胱甘肽(GSH)、抗坏血酸(AsA)、脱氢抗坏血酸(DHA)等抗氧化物的量有高于园参的趋势。比较谷胱甘肽合成前体的上游氨基酸(谷氨酸、半胱氨酸、甘氨酸)量,发现野山参亦高于园参,保障了AsA-GSH再循环的平衡。结论 AsA-GSH循环中关键抗氧化酶活性及抗氧化物量均为野山参高于园参,导致野山参抗氧化能力强于园参,为野山参与园参功效差异研究提供了理论依据。

Comparison on difference of ascorbic acid-glutathione cycle between wild and cultivated ginseng

Objective In order to explore the anti-oxidant capacity of wild and cultivated ginseng, the anti-oxidase activities and antioxidants contents on ascorbic acid-glutathione(AsA-GSH) cycle were compared. Methods The activity of superoxide dismutase(SOD) was detected by NBT method and the activity of catalase(CAT) was detected by potassium permanganate titration. The anti-oxidase activities and anti-oxidants contents on AsA-GSH cycle were tested using spectrophotometric determination. The contents of glutamate(Glu), cysteine(Gly), and glycine(Cys) were tested by automatic amino acid analysis. Results The activities of SOD and CAT in wild ginseng were higher than that of cultivated ginseng. In AsA-GSH cycle, the activities of anti-oxidase such as ascorbate peroxidase(APX), monodehydroascorbate reductase(MDHAR), and glutathione reductase(GR). And the contents of antioxidants such as glutathione(GSH), ascorbic acid(AsA), and dehydroascorbic acid(DHA) were also more in wild ginseng. And then the contents of amino acids(Glu, Gly, and Cys), the precursor of GSH synthesis were high expression in wild ginseng to keep the balance of AsA and GSH recirculation. Conclusion The anti-oxidase activities and antioxidants content on AsA-GSH cycle in wild ginseng are higher than those in cultivated ginseng, which may be leaded to the antioxidant capacity of wild ginseng is stronger. It will provide a theoretical basis for efficacy differences research on wild and cultivated ginseng.