| 乙肝病毒复制是慢性肝病活动的直接原因 |
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| 引用本文： | 张智,骆抗先,梁炽森.乙肝病毒复制是慢性肝病活动的直接原因[J].南方医科大学学报,1994(1). |
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| 作者姓名： | 张智 骆抗先 梁炽森 |
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| 作者单位： | 南方医院传染科 |
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| 摘 要： | 聚合酶链反应（ＰＣＲ）检测４８例ＨＢｓＡｇ（＋），抗ＨＢｅ（＋）慢性肝病患者血清ＨＢＶＤＮＡ，３８例阳性，１０例阴性．１０例ＰＣＲ阴性产物用狄高辛素标记探针进行斑点杂交，又检出６例ＨＢＶＤＮＡ阳性，ＨＢＶＤＮＡ总阳性率为９２％，ＨＢＶＤＮＡ阳性患者丙氨酸转氨酶（ＡＬＴ）水平均增高，而阴性患者ＡＬＴ在正常水平。结果表明，此类病人体内存在ＨＢＶ复制，导致病变持续活动甚至加重。
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| 关 键 词： | HBVDNA，抗HBe，聚合酶链反应 |
HBV replication as a cause of reactivity of Chronic liver diseases |
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| Zhang Zhi,Luo
Kangxian,Liang Zhisen.HBV replication as a cause of reactivity of Chronic liver diseases[J].Journal of Southern Medical University,1994(1). |
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| Authors: | Zhang Zhi Luo
Kangxian Liang Zhisen |
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| Institution: | Zhang Zhi,Luo
Kangxian,Liang Zhisen Department of Infectious Diseases,Nanfang Hospital,First Military
Medical,Guangzhou 510515 |
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| Abstract: | Serum HBV DNA was investigated with polymerase chain
reaction(PCR ) in 48 cases of chronic liver diseases with HBsAg and anti-HBe positive of them
38 were positive and 10 negative for HBV DNA. The HBV DNA negative PCR products were
further tested with dot-blot hybridization using a digoxigen labeled probe and HBV DNA was
detected in another 6 cases. So the total HBV DNA prevalence was 92%. 10 selected
patients,were tested both before and after anti-HBe seroconversion , and itwas found that HBV
DNA was positive in all 10 cases by PCR technique both before and after seroconversion.
Alanine transaminase level was increased in all HBV DNA positive patients, while it was normal
in HBV DNA negative patients. The results show that HBV replication might be the cause of
reactivity of chronic liver diseases. |
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| Keywords: | HBV DNA anti-HBe polymerase chain reaction(PCR) |
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