乌司他丁对心肺复苏后大鼠脑组织含水量及CD11b的影响

目的 探讨乌司他丁对心肺复苏早期大鼠脑组织含水量及脑组织CD11b水平的影响.方法 将30只SD大鼠随机分为假手术对照组、心肺复苏(CPR)组、乌司他丁组,每组各10只大鼠.于自主呼吸循环恢复后2h,采用干湿法检测各组大鼠脑组织含水量,采用ELISE法检测大鼠脑组织CD11b水平.结果 ①脑组织含水量:CPR组为(80.4±2.0)%,较对照组(76.7±1.3)%显著增加(P<0.01);乌司他丁组(77.6±1.5)%,较CPR组显著减少(P<0.01).②脑组织CD11b含量:CPR组为(620.488±38.723)ng/mg,较对照组(453.189±121.403)ng/mag明显增高(P<0.01);乌司他丁组(476.875±114.686)ng/mg,较CPR组显著减少(P<0.05).结论 CPR早期即可发生脑水肿,脑组织CD11b水平显著增加;应用乌司他丁能有效降低脑组织含水量和脑组织CD11b水平.

The Effects of UTI on Brain Edema and CD11b in Brains of Rats after CPR

Objective To observe the effects of UTI on the moisture capacity and CD11b of brains in CPR rats. Methods 30 grown male Sprague Dawley(SD) rats were divided randomly into three groups :control group,resuscitation group and UTI group. Each group had 10 rats. The last two groups were used to make CPR models induced by asphyxia and were observed lasting 2 hours after ROSC. Then all rats were caused dead and brain tissues were took out. Brain tissues were weighed (humid weight),then they were dried in a baker and scaled dry weight. Moisture capacities were calculated by Elliott method. The lever of CD11b in brain tissue was determined by ELISA method. Results ①The brain water content in the resuscitation group was significantly higher than that in the control group(80.4 ± 2.0)% vs (76.7 ± 1.3)%, P < 0.01];The brain water content in UTI group was decreased significantly than that in the resuscitation group(77.6 ± 1.5)% vs (80.4 ± 2.0)%, P<0.01]. ②The levels of CD11b of brain tissues in the resuscitation group increased significantly than those in the control group (620.488 ± 38.723) ng/mg vs (453.189 ± 121.403) ng/mg, P < 0.01]; The levels of CD 11 b of brain tissues in UTI group decreased significantly than those in the resuscitation group(476.875 ± 114.686)ng/mg vs (620.488 ± 38.723)ng/mg, P < 0.05]. Conclusion Brain edema occurs during the early stage of CPR,and the levers of CD11b in brain tissues increase significantly. The use of UTI may effectively reduce the brain water content,the levels of CD11b in brain tissues.