Nestin immunoreactivity in local neurons of the adult rat striatum after remote cortical injury |
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Authors: | Kuroda Tatsuya Nakamura Hiroyuki Itoh Kazuo Le Wu Ri Yoshimura Shin-ichi Takenaka Katsunobu Sakai Noboru |
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Institution: | Department of Neurosurgery, Gifu University School of Medicine, Japan. |
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Abstract: | Nestin is a marker for the neuronal and glial precursor cells and is expressed in reactive astrocytes after brain injury. Following restricted neocortical injury, we found that cells with neuronal morphology in the adult rat striatum became immunoreactive for both nestin and the neuronal marker, microtubule-associated protein 2 (MAP-2), but not for the astroglial marker, glial fibrillary acidic protein (GFAP). The number of nestin-positive cells transiently increased in the striatum. Continuous administration of 5-bromo-2'-deoxyuridine (BrdU) after cortical injury did not reveal any newly generated neurons in the striatum. Double-labeling fluorescent immunocytochemistry revealed that the nestin-positive striatal cells were also substance-P-positive. These findings suggest that some factors released from the injured cortex may induce nestin immunoreactivity in striatal neurons. |
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Keywords: | Neural stem cells Caudate-putamen Basal ganglia MAP-2 GFAP Substance-P BrdU |
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