Folate-mediated poly(3-hydroxybutyrate-co-3-hydroxyoctanoate) nanoparticles for targeting drug delivery |
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| Authors: | Chan Zhang LiangQi Zhao YueFeng Dong XiaoYan Zhang Ji Lin Zhang Chen |
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| Institution: | 1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;2. School of Materials Science and Engineering, Jilin Jianzhu University, Changchun 130118, China;1. Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan;2. Department of Metallurgy and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan;1. School of Pharmacy, Faculty of Health & Medical Sciences, Taylor''s University, Subang Jaya, Malaysia;2. Department of Pharmaceutical Technology, School of Pharmacy, International Medical University, Bukit Jalil, Malaysia;3. Department of Pharmaceutics, Sinhgad Technical Education Society''s, Sinhgad Institute of Pharmacy (Affiliated to Savitribai Phule Pune University), Narhe, Pune, India;6. Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, Delhi, India |
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| Abstract: | A novel targeting drug delivery system (TDDS) has been developed. Such a TDDS was prepared by W1/O/W2 solvent extraction/evaporation method, adopting poly(3-hydroxybutyrate-co-3-hydroxyoctanoate) P(HB-HO)] as the drug carrier, folic acid (FA) as the targeting ligand, and doxorubicin (DOX) as the model anticancer drug. The average size, drug loading capacity and encapsulation efficiency of the prepared DOX-loaded, folate-mediated P(HB-HO) nanoparticles (DOX/FA–PEG–P(HB-HO) NPs) were found to be around 240 nm, 29.6% and 83.5%. The in vitro release profile displayed that nearly 50% DOX was released in the first 5 days. The intracellular uptake tests of the nanoparticles (NPs) in vitro showed that the DOX/FA–PEG–P(HB-HO) NPs were more efficiently taken up by HeLa cells compared to non-folate-mediated P(HB-HO) NPs. In addition, DOX/FA–PEG–P(HB-HO) NPs (IC50 = 0.87 μM) showed greater cytotoxicity to HeLa cells than other treated groups. In vivo anti-tumor activity of the DOX/FA–PEG–P(HB-HO) NPs showed a much better therapeutic efficacy in inhibiting tumor growth, and the final mean tumor volume was 178.91 ± 17.43 mm3, significantly smaller than normal saline control group (542.58 ± 45.19 mm3). All these results have illustrated that our techniques for the preparing of DOX/FA–PEG–P(HB-HO) NPs developed in present work are feasible and these NPs are effective in selective delivery of anticancer drug to the folate receptor-overexpressed cancer cells. The new TDDS may be a competent candidate in application in targeting treatment of cancers. |
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