儿童RRTIsT细胞亚群和NK细胞的变化分析

目的了解3～6岁儿童反复呼吸道感染（RRTIs）T细胞亚群及自然杀伤细胞（NK）的变化，指导临床预防用药，避免过度以及不当治疗。方法严格按照入选标准和剔除标准收集3～6岁儿童160例，其中：病例组73例，对照组87例。采用病例对照研究。用流式细胞仪对病例组和对照组分别进行T细胞亚群、NK细胞的检测，并对有关数据进行统计分析。结果对T细胞亚群（CD3＋、CD4＋、CD8＋、CD4＋／CD8＋）进行两独立样本的Wilcoxon秩和检验，结果表明：CD3＋（Z=-1．588，P=0．112）、CD4＋（Z=-0．541，P=0．588）、CD8＋（Z=-0．733，P=0．463）、CD4＋／CD8＋（Z=-0．315，P=0．753）病例组和对照组比较差异均无统计学意义（均P〉0．05）。对NK细胞（CD3-CD16＋CD56＋）进行t＋检验（t＇=2．779，P=0．006〈0．05；95％CI：0．674～4．003），病例组和对照组比较差异有统计学意义。结论对RRTIs儿童检测T细胞亚群与NK细胞具有显著的临床指导意义，可避免过度使用免疫调节剂引起的用药不当。

Analysis of the variations of T cell subset and NK cells in children with RRTIs

Objective To investigate the variations of T cell subset and NK cells in children aged 3-6 years old with recurrent respiratory tract infections （RRTIs） , so as to provide new guidance for clinical preventive medication and avoid unreasonable therapy. Methods In a case-control study, 160 children aged 3-6 years old were selected strictly according to criterion, including 73 cases in case group and 87 cases in control group. The T cell subset and NK cells from peripheral blood of two groups were measured by flow cytometry method for data analysis. Results Wilcoxon rank-sum test on T cell subset showed that there was no significant difference between two groups in CD3 ＋ （z = -1.588, P=0.112）, CD4 ＋（z= -0.541,P=0.588）, CD8 ＋（z= -0.733, P=0.463） andCD4 ＋/CD8 ＋（z= -0.315, P=0.753） （all P 〉0.05）. But there was significant difference in NK cells between two groups （t＇ = 2. 779, P = 0. 006 〈 0.05; 95% CI： 0. 674- 4. 003 ）. Conclusion Detecting T cell subset and NK cells in children with RRITs is very useful in clinics, which can avoid unreasonable medication caused by excessive immunoregulant.