Oscillatory Perfusion Culture of CaP-Based Tissue Engineering Bone with and without Dexamethasone |
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Authors: | Dajiang Du Katsuko S Furukawa and Takashi Ushida |
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Institution: | (1) Laboratory of Biomedical Engineering, Department of Mechanical Engineering, Graduate School of Engineering, University of Tokyo, 2nd Building, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;(2) Department of Bioengineering, Graduate School of Engineering, University of Tokyo, 2nd Building, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;(3) NanoBio Integration, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-8656, Japan;(4) Division of Biomedical Materials and Systems, Center for Disease Biology and Integrative Medicine, School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan |
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Abstract: | Dexamethasone, a powerful osteogenic agent for osteoblast differentiation, has been suggested to have synergistic effects
when applied together with perfusion culture. As ceramic scaffolds are widely used clinically and oscillatory flow well replicates
the natural physical conditions, the biological effects of dexamethasone on oscillatory perfusion culture of CaP-based tissue
engineering bone were investigated in this study. Mouse osteoblast-like cells, MC 3T3-E1, were seeded onto porous ceramic
scaffolds using the oscillatory perfusion method. The seeded constructs were then either cultured by a static method or an
oscillatory perfusion method at different flow rates continuously for 6 days with and without dexamethasone. The cell proliferation,
early osteogenic effects, and viability were subsequently evaluated. The results showed that the oscillatory flow could enhance
early osteogenesis of osteoblast-like cells in three-dimensional culture on ceramic scaffolds, with a peak function at the
flow rate of 0.5 mL/min. The cell viability was significantly higher and more uniform in the perfusion groups than in the
static culture groups. The uniformity decreased as the perfusion rates decreased. However, dexamethasone seems to have had
no significant effects in any of the groups. Our results suggest that dexamethasone is not an efficient osteogenic supplement
during perfusion culture on CaP ceramic scaffolds, and predifferentiation before seeding or additional osteogenic factors
should be considered for such cultures. |
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Keywords: | Flow perfusion Bioreactor Dexamethasone Ceramic scaffold Bone tissue engineering |
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