| 小白菊内酯对白血病K562细胞及其干细胞的作用 |
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| 引用本文： | 易娟,陈静,孙静,魏虎来,石建功.小白菊内酯对白血病K562细胞及其干细胞的作用[J].中国中药杂志,2010,35(2):219-222. |
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| 作者姓名： | 易娟 陈静 孙静 魏虎来 石建功 |
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| 作者单位： | 1. 兰州大学,基础医学院,医学实验中心,甘肃省新药临床前研究重点实验室,甘肃,兰州,730000
2. 北京协和医学院,中草药物质基础与资源利用教育部重点实验室,北京,100005 |
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| 基金项目： | 甘肃省自然科学研究基金项目(096RJZA034);中草药物质基础与资源利用教育部重点实验室(北京协和医学院)开放课题(0801);甘肃省新药临床前研究重点实验室(兰州大学)开放课题(GSKFKT-0802) |
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| 摘 要： | 目的:研究小白菊内酯(parthenolide,PTL)对白血病K562细胞及其白血病干细胞(leukemia stem cells,LSC)的作用.方法:以白血病K562细胞为靶细胞,四氮唑蓝(MTT)比色法测定细胞增殖活性,Annexin V/PI染色法测定细胞凋亡;流式细胞术检测LSC相对含量,甲基纤维素集落形成法检测细胞的自我更新和增殖能力.结果:PTL显著抑制K562细胞的增殖,24,48,72 h的IC_(50)分别为17.1,8.67,9.42μmol·L~(-1).5,10 μmol·L~(-1)PTL处理48 h,K562细胞的凋亡率分别为(49.56±5.11)%,(71.88±2.12)%.结合干细胞免疫标志分析,K562细胞中LSC样(CD34~+ CD38~-)细胞的凋亡率分别为(52.63±4.14)%,(57.50±4.47)%.K562细胞中LSC的相对含量仅轻度增高,但高浓度(15 μmol·L~(-1))PTL处理,LSC含量则增高15倍.0.5～4.0 μmol·L~(-1)PTL显著抑制K562细胞的集落形成能力,集落数降低24.1%～89.2%;5～15 μmol·L~(-1)PTL预处理,存活K562细胞的集落形成数增高5.0%～50.0%.结论:小白菊内酯可抑制K562细胞及其干细胞的增殖活性,并诱导其凋亡.
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| 关 键 词： | 白血病干细胞 小白菊内酯 集落形成 凋亡 |
| 收稿时间： | 2009/3/12 0:00:00 |
Effect of parthenolide on leukemia K562 cells and its leukemia stem cells |
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| YI Juan,CHEN Jing,SUN Jing,WEI Hulai and SHI Jiangong.Effect of parthenolide on leukemia K562 cells and its leukemia stem cells[J].China Journal of Chinese Materia Medica,2010,35(2):219-222. |
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| Authors: | YI Juan CHEN Jing SUN Jing WEI Hulai and SHI Jiangong |
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| Institution: | Laboratory Center for Medical Science, School of Medicine, Lanzhou University, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 73000, China;Laboratory Center for Medical Science, School of Medicine, Lanzhou University, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 73000, China;Laboratory Center for Medical Science, School of Medicine, Lanzhou University, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 73000, China;Laboratory Center for Medical Science, School of Medicine, Lanzhou University, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou 73000, China;Peking Union Medical College, Beijing 100005, China |
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| Abstract: | Objective: To investigate the inhibitory and apoptosis-inducing effects of parthenolide(PTL) on human leukemia K562 cells and its leukemia stem cells(LSC).Method: MTT assay was used to detect the proliferating activity of K562 cells,and the cellular apoptosis was assayed with Annexin V/PI double staining.Flow cytometry(FCM) was employed to determine the relative proportion of LSC in K562 cells.The self-renewal and proliferating potential were examined with methylcellulose colony-forming units(CFU) assay.Res... |
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| Keywords: | leukemia stem cells parthenolide colony formation apoptosis |
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