RNA指纹法分离血管性痴呆大鼠海马内的差异表达基因

目的：分离血管性痴呆（VD）大鼠海马内疾病相关基因，方法：用改良的Pulsinelli-4血管阻断全脑缺血法4－VO法建立VD模型，Morris水迷宫检测其痴呆，以RNA指纹法对比正常老龄大鼠和血管性痴呆大鼠海马组织基因表达，并分离疾病情况下表达差异的基因。结果：在成功建立VD大鼠模型的基础上，分离、筛选、克隆到32条VD海马内疾病相关差异表达基因片段，选取2条VD特异表达的片段进行Northern杂交验证，通过测序与GenBank比较证均为新基因片段，登录号为BG937392、BG937393，结论：RNA指纹法是快速、简便，有效的分离差异基因的方法，本实验分离的差异表达基因可能是直接参与疾病发生，发展过程的致病基因或保护基因。

Isolation of differently expressed cDNAs from the hippcampus of rats with vascular dementia by RNA fingerprinting

Objective To isolate differently expressed cDNAs associated with vascular dementia from hippocampus of rats. Methods The creation of vascular dementia model of rat was by 4 vessel occlusion method. The learning and memory of vascular dementia(VaD) rats were examined by Morris water maze. RNA fingerprinting was used to analyse the differently expressed cDNAs in the hippocampus of normal aging and VaD rats. At the same time, cDNAs expressed differently during the progression of VaD were isolated. Results Thirty two candidate cDNA fragments were isolated by RNA fingerprinting. The two cDNA fragments specifically expressed in VaD rats were analysed by Northern blot. Homology analysis through BLAST revealed that these two were novel genes and were given numbers of BG937392 and BG937393 in the gene bank. Conclusions RNA fingerprinting is a simple and quick method for isolating differently expressed genes. These two new gene fragments may contribute significantly to the process of VaD as pathogenic genes or protective genes.