环孢素A抑制丙型肝炎病毒JFH-1复制的实验研究

目的 探讨环孢素A(cyclosporine A,CsA)对全基因组丙型肝炎病毒JFH-1(hepatitis C virus,HCV JFH-1)在肝细胞中复制的影响.方法 以体外转录的HCV JFH-1 RNA转染Huh7细胞,制备含HCV病毒颗粒的感染上清,建立全基因组HCV JFH-1感染Huh7细胞模型,分为...

Effects of Cyclosporine A on Hepatitis C Virus Replication in Human Hepatocytes

Objective To investigate the effect of cyclosporine A（CsA） on full cycle hepatitis C virus（HCV） JFH-1 replication in human hepatocytes.Methods Infectious HCV JFH-1 generated in Huh7 cells by transfection of in vitro transcribed genomic JFH1 RNA was used to infect naive Huh7 cells.The infected cells were treated by CsA with different concentrations,time courses and strategies.HCV replication was determined by real time polymerase chain reaction（RT-PCR）.Results After CsA（0.15,0.3,0.6,1.2 μg/ml） treatment,the relative HCV JFH-1 RNA expression compared with the control group decreased to（45.7±6.5）%,（18.9±2.1）%,（4.6±0.7）%,（2.1±0.2%） and HCV copies（×10^7/ml） in the supernatant decreased to（0.80±0.19）、（0.44±0.07）,（0.29±0.07）,（0.17±0.06） respectively.In the CsA group,the HCV protein synthesis,virus contents in the supernatant,and the infectivity of Hun7 cell lysis solution and supernatant were significant decreased.Furthermore,CsA preprocessing inhibited the HCV JFH-1 better than CsA treatment during and after infection,and CsA withdrawal resulted in significant virus replication reoccurrence.Conclusion CsA can effectively inhibit the full cycle HCV JFH-1 replication in Huh7 cells and reduce production of infectious HCV virus.