人参皂甙Rd联合远程预处理对脑缺血大鼠的脑保护效应

目的 比较单独或联合应用人参皂甙Rd及远程预处理的脑保护效应,探寻安全有效的脑保护方法.方法 40只雄性SD大鼠随机均分为4组(n=10/组):①对照组(C组),腹腔注射生理盐水1 h后制备大脑中动脉栓塞-再灌注(middle cerebral artery occlusion-reperfusion,MCAO)模型;...

Neuroprotective Effect of Combined Ginsenoside Rd with Remote Preconditioning after Focal Cerebral Ischemia in Rats

Objective To investigate the neuroprotective effect of the combined ginsenoside Rd with remote preconditioning in rats and seek the safe and effective neuroprotective method.Methods Forty male SD rats were randomly assigned to four groups（n=10 each）.In the control group（group C）,the rats were intraperitoneally injected with saline 1 hour before middle cerebral artery occlusion-reperfusion（MCAO） was performed.In the ginsenoside Rd group（group Rd）,the rats were intraperitoneally injected with ginsenoside Rd（30 mg/kg） 1 hour before MCAO.In remote preconditioning group（group RPC）,the rats received remote preconditioning（RPC） 1 hour before MCAO.In combined ginsenoside Rd with remote preconditioning group（group Rd＋RPC）,the rats were intraperitoneally injected with ginsenoside Rd（30 mg/kg） 1 hour before RPC and MCAO was performed 1 hour after RPC.The neurological behavior scores（NBS） were assessed at 24 and 72 hour after reperfusion respectively,and the percentage of infarct volume was determined using 2,3,5-triphenyltetrazolium chloride（TTC） staining at 72 hours after reperfusion.Results The NBS in three preconditioning groups were better than that in group C（P〈0.01）,in group RPC was better than in group Rd,and in group Rd＋RPC was better than in groups Rd and RPC respectively（P〈0.05）.The percentage of infarct volume in three preconditioning groups were significantly lower than that in group C（P〈0.01）,in group RPC was lower than in group Rd,and in group Rd＋RPC was lower than in groups Rd and RPC respectively（P〈0.05）.Conclusion Ginsenoside Rd and remote preconditioning have significantly neuroprotective effects in rats,and their combination exhibits synergistic protective effect.