Bay41-4109抗乙型肝炎病毒的体外实验研究

目的 观察Bay41-4109对2.2.15细胞分泌乙型肝炎病毒表面抗原(HBsAg)、乙型肝炎病毒e抗原(HBeAg)和乙型肝炎病毒(hepatitis B virus,HBV)DNA的影响,探讨Bay41-4109的体外抗HBV作用.方法 将一定浓度的Bay41-4109加入培养的2.2.15细胞,通过四甲基偶氮唑...

Inhibitory Effects of Bay41-4109 on Hepatitis B Virus in vitro

Objective To investigate the anti-hepatitis B virus（HBV） effect of Bay41-4109 on the secretion of HBsAg,HBeAg,HBV DNA in 2.2.15 cells.Methods 2.2.15 cells were cultured and treated with different concentrations of Bay41-4109 for 6 days.The cytotoxicity activity of drugs was determined by methyl thiazolyl tetrazolium（MTT） colorimetric assay,the concentrations of HBsAg and HBeAg in the culture supernatant were detected by real-time enzyme-linked immunosorbent assay（ELISA）,and the extracellular HBV DNA was measured by real-time polymerase chain reaction（RT-PCR）.Results The toxicity of Bay41-4109 on 2.2.15 cells increased from 12.5 to 50 μM with TC50 of 35.43 μM and TC0 of 12.50 μM.Bay41-4109 had dose-dependent inhibition effect on HBeAg secretion with IC50 of 8.22 μM.The secretion of HBsAg was not inhibited 6 days after Bay41-4109 treatment.The extracellular HBV DNA level of 2.2.15 cells was significantly reduced after Bay41-4109 treatment,and this inhibitory effect was not dose-dependent.Conclusion Bay41-4109 can inhibit the secretion of HBeAg and replication of HBV DNA in 2.2.15 cells.