| 可溶性趋化因子对人单核细胞表达及基质金属蛋白酶-9的影响 |
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| 引用本文： | 李政,孙子林,王黎,方铭. 可溶性趋化因子对人单核细胞表达及基质金属蛋白酶-9的影响[J]. 中国微循环, 2009, 13(5): 341-343 |
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| 作者姓名： | 李政 孙子林 王黎 方铭 |
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| 作者单位： | 东南大学附属中大医院内分泌科,江苏南京,210009 |
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| 摘 要： | 目的观察趋化因子Fractalkine（CX3CL1,Fkn）对人单核细胞株U937细胞表达与分泌基质金属蛋白酶-9（matrix metalloproteinase-9,MMP-9）的影响。方法使用不同浓度的人重组Fkn干预U937细胞,RT-PCR法检测细胞MMP-9基因表达,明胶酶谱法检测细胞上清中MMP-9水平。结果不同浓度人重组Fkn干预U937细胞MMP-9基因表达及上清中MMP-9水平低于空白对照组。结论Fkn可抑制U937细胞表达与分泌MMP-9。
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| 关 键 词： | 可溶性趋化因子 基质金属蛋白酶-9 人单核细胞 糖尿病肾病 |
Effect of Fractalkine on the Expression and Secretion of MMP-9 in Human Monocytes |
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| LI Zheng,SUN Zi-Lin,WANG Li,FANG Ming. Effect of Fractalkine on the Expression and Secretion of MMP-9 in Human Monocytes[J]. Journal of Chinese Microcirculation, 2009, 13(5): 341-343 |
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| Authors: | LI Zheng SUN Zi-Lin WANG Li FANG Ming |
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| Affiliation: | . (Department of Endocrinology, Zhong Da Hospital, Southeast University, Nanjing , 210009,China) |
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| Abstract: | Objective To study the effect of Fractalkine （ CX3CL1, Fkn） on the expression and secretion of matrix metalloproteinase-9 （MMP-9） in cultured U937 cells （ human monocyte line）. Methods The cultured U937 cells were incubated with recombinant human Fkn for 24 h. The mRNA expression of MMP-9 was analyzed by RT-PCR. The production of MMP-9 in the supernatant was analyzed by gelatin zymography. Results The level of MMP-9 mRNA and production in the cells incubated with recombinant human Fkn decreased compared with control group. Conclusion Fkn can inhibit the expression and secretion of MMP-9 in cultured U937 cells. |
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| Keywords: | Fractalkine Matrix metalloproteinase-9 Human monocyte Diabetic nephropathy |
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