| 人神经营养因子—3在哺乳动物细胞中瞬时表达和稳定表达的初步研究 |
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| 引用本文： | 欧阳立明 周涛 等. 人神经营养因子—3在哺乳动物细胞中瞬时表达和稳定表达的初步研究[J]. 医学教育探索, 2001, 0(3): 258-260 |
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| 作者姓名： | 欧阳立明 周涛 等 |
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| 作者单位： | [1]华东理工大学生物反应器工程国家实验室，上海200237 [2]军事医学科学院生物工程研究所，北京100071 |
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| 摘 要： | 将人神经营养因子－3全长cDNA克隆入真核表达载体pcDNA3,分别COS-7儿CHO细胞中获得了瞬时表达和稳定表达，经大乳鼠脊髓背根神经节测活，瞬时表达和稳定表达产物均能明显促进其神经突起的生长，瞬时表达量约为10^-7g/mL，稳定表达量可达10^-8g/mL。
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| 关 键 词： | 人神经营养因子－3 真核表达载体pc DNA3 背根神经节 瞬时表达 稳定表达 神经生长 |
Initial Research for Transient and Stable Expression of Human Neurotrophin-3 in Mammalian Cells |
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| OUYANG Li ming,ZHOU Tao,XUE Chong,LIU Zhi min,ZHANG Si liang. Initial Research for Transient and Stable Expression of Human Neurotrophin-3 in Mammalian Cells[J]. Researches in Medical Education, 2001, 0(3): 258-260 |
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| Authors: | OUYANG Li ming ZHOU Tao XUE Chong LIU Zhi min ZHANG Si liang |
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| Abstract: | Human neurotrophin 3 (hNT 3) cDNA encoding preproNT 3 was inserted into pcDNA3, an eukaryocyte expression vector. The reconstituted plasmid was expressed in COS 7 cell transiently and CHO cell steadily. Tested by dorsal root ganglion of rat spinal cord, the product could promote the outgrowth of the neurite obviously. |
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| Keywords: | hNT 3 pcDNA3 dorsal root ganglion transient expression stable expression |
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