| 32例有偿献血感染者HIV-1膜区序列测定及V3环氨基酸变异特点分析 |
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| 引用本文： | 代丽丽,鲁俊峰,黄晓婕,石英,陈德喜,吴昊.32例有偿献血感染者HIV-1膜区序列测定及V3环氨基酸变异特点分析[J].中华实验和临床病毒学杂志,2008,22(1):27-29. |
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| 作者姓名： | 代丽丽 鲁俊峰 黄晓婕 石英 陈德喜 吴昊 |
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| 作者单位： | 首都医科大学附属北京佑安医院感染科 |
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| 基金项目： | 北京市科委科技计划项目，国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的 了解我国有偿献血人群中HIV-1B'亚型流行株的膜蛋白V3环序列特征.方法 巢式PCR扩增前病毒DNAc2-c3区后测序,进行病毒亚型鉴定和V3环序列特点分析.结果 所检的32个HIV-1B'亚型株V3环顶端四肽有五种形式.AIDS组与无症状感染组相比前者呈现更多的T/SI型毒株V3环变异特点.结论 我国有偿献血人群中HIV-1 B'亚型流行株V3环顶端四肽有多种形式.处于病程不同时期的患者体内病毒V3区呈现不同的氨基酸变异特点和电荷积累趋势,提示可能具有不同的生物学表型特征.
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| 关 键 词： | 基因型 序列分析 生物学表型 |
Analysis of the variation of 32 HIV-1 V3 loop from infected Chinese blood/plasma donors |
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| DAI Li-li,LU Jun-feng,HUANG Xiao-jie,SHI Ying,CHEN De-xi,WU Hao.Analysis of the variation of 32 HIV-1 V3 loop from infected Chinese blood/plasma donors[J].Chinese Journal of Experimental and Clinical Virology,2008,22(1):27-29. |
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| Authors: | DAI Li-li LU Jun-feng HUANG Xiao-jie SHI Ying CHEN De-xi WU Hao |
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| Institution: | Department of Infectious Disease. The You An Hospital affiliated to The Capital University of Medical Science. Beijing, 100069, China. |
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| Abstract: | OBJECTIVE: To characterize the variation in V3 loop of HIV-1 B'strains circulating in Chinese blood donors. METHODS: The c2-c3 regions of the HIV envelop gene were amplified by nest-PCR from 32 HIV-1-infected blood donors in He Nan province in China. The BIOEDIT and MEGA software are used to analyze the sequences of V3 loop. RESULTS: There are five types of central motifs of the 32 samples, in which GPGR and GPGQ are most common. More variations associated with T tropic/SI phenotype can be seen in AIDS group. CONCLUSION: The V3 tip motifs of HIV-1B' strains circulating in Chinese blood donors are various, the different characterization of V3 loop between AIDS and asymptomatic patients indicates different biological phenotype and pathogenesis which warrant additional investigation. |
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| Keywords: | HIV-1 |
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