一氧化氮诱导VSMC凋亡观察

目的研究动脉粥样硬化和冠脉气囊成形术后再狭窄中血管平滑肌细胞数量异常增多的机制，并初步探讨ＮＯ诱导血管平滑肌细胞凋亡的作用。材料和方法　以ＳＮＡＰ作为ＮＯ供体，采用ＨＥ，ＴＵＮＥＬ，荧光双染后光镜观察以及电镜、流式细胞术、细胞ＤＮＡ的琼脂糖凝胶电泳对ＳＮＡＰ作用下的细胞凋亡进行鉴定和检测。结果　观察发现， ０．４ｍｍｏｌ／Ｌ　 ＳＮＡＰ作用 ８－１０ ｈ即能明显地诱导培养的血管平滑肌细胞凋亡。结论 ＳＮＡＰ以浓度依赖方式诱导血管平滑肌细胞凋亡；细胞爬片的ＨＥ染色结合ＴＵＮＥＬ标记是检测凋亡的较为准确且简单的方法。

Nitric oxide induced apoptosis in vascular smooth muscle cells

Objective To further study the significant number increase of vascular smooth muscle cells in atherosclerosis and resterosis, We investigated the effect of NO on the induction of apoptosis in cultured vascular smooth muscle cells. Methods SNAP was used as NO donor. HE, TUNEL, double fluorescent staining and electronic microscopy, flow cytometry, agarose gel electrophoresis of DNA were employed to detect and observe cell apoptosis, Result Vascular smooth muscle cells showed apoptosis when treated with 0.4 mmol/L SNAP for 8 to 10 hours, Conclusions The results indicated that SNAP dosedependently induce apoptosis in VSMC. and HE staining along with TUNEL was a simple method to observe and detect apoptosis in ceels is cultured on slide.