五倍子酸对辐射损伤小鼠造血系统的防护作用

目的 研究中药五倍子的主要有效成分五倍子酸对⁶⁰Co-γ射线诱导的小鼠造血系统损伤的防护作用，并对其作用机制进行初步探讨。方法 实验动物选用ICR小鼠和BALB/c小鼠。分为正常对照组、辐照组、五倍子酸低剂量组（100 mg/kg GA+辐照）、五倍子酸高剂量组（200 mg/kg GA+辐照）和阳性对照组（WR-2721+辐照）共5组。观察五倍子酸对电离辐射诱导的小鼠脾重、脾系数、脾结节形成能力、外周血中WBC、RBC、LYM、PLT数目以及脾脏中凋亡相关蛋白Bax、Bcl-2、P53含量和凋亡相关激酶Caspase3变化的影响。结果 100 mg/kg 和200 mg/kg的五倍子酸能显著减轻辐照所致小鼠脾重下降的程度（P<0.05），200 mg/kg的五倍子酸能显著改善脾系数下降的程度，并能明显提高受照小鼠脾结节形成能力（P<0.05）。100 mg/kg 和200 mg/kg的五倍子酸能抑制小鼠外周血中RBC、WBC下降的程度，并能明显改善辐射诱导的PLT含量减少（P<0.05）。100 mg/kg 和200 mg/kg的五倍子酸能显著降低小鼠脾脏细胞Bax/Bcl-2 的比值（P<0.05），但并没有引起p53 和Caspase3水平显著下降（P>0.05）。结论 五倍子酸对辐射所致小鼠损伤具有一定防护作用，其作用机制可能与保护造血系统和减轻细胞凋亡有关。

Protective-effects of gallic acid on ionizing irradiated mice in hematopoietic system

Objective To investigate protective effects of gallic acid on ⁶⁰Co-γ ray radiation induced hematopoietic system injuries of mice. Methods ICR mice and BALB/c mice, divided into 5 groups:control group, ray radiation group, 100 mg/kg GA+ γ ray radiation group,200 mg/kg GA+γ ray radiation group,WR-2721+γ ray radiation group. Observed the spleen weights,spleen indexes, CFU-spleen,RBC, WBC,LYM,PLT counts in the peripheral blood of mice and the expression level of Bax,Bcl-2,P53 and Caspase3 in spleen of mice were observed too. Results GA(100 mg/kg,200 mg/kg)could decreased the injury of the spleen weights, GA(200 mg/kg)could increased the spleen indexe and colony forming units induced by ⁶⁰Co-γ ray irradiation compared with the control group significantly(P<0.05). GA(100 mg/kg,200 mg/kg) could weakend the the injury of RBC,WBC,PLT counts in blood induced by ⁶⁰Co-γ ray irradiation significantly (P<0.05). GA(100 mg/kg,200 mg/kg) could reduced the rate of Bax/Bcl-2 significantly (P<0.05), but have no significant on the expression of p53 and Caspase3(P>0.05). Conclusion GA has protective effect on ⁶⁰Co-γ ray radiation induced mice injuries; the mechanism may be related to the reduction of hematopoietic system injury and cell apoptosis induced by irradiation.