合并感染华支睾吸虫对慢性乙肝患者肠道菌群的影响

目的 了解慢性乙肝患者合并华支睾吸虫感染后肠道菌群的生长状况,探索肠道菌群与合并感染状态的相互关系,从而为临床预防和治疗提供帮助。方法 运用稀释平板菌落计数法,收集健康人、单纯患慢性乙肝的患者、单纯感染华支睾吸虫的患者、慢性乙肝合并华支睾吸虫感染者4组人群的新鲜大便标本各30例进行培养,并以肠杆菌、肠球菌、乳酸杆菌以及双歧杆菌四种菌群为研究对象进行统计分析,比较4组人群肠道菌群的变化,分析合并感染华支睾吸虫对慢性乙肝患者肠道菌群的影响。结果 与健康对照组相比,单纯患慢性乙肝的患者组和单纯感染华支睾吸虫的患者组肠杆菌、肠球菌数量增加,乳酸杆菌、双歧杆菌数量减少,差异具有统计学意义（P<0.05）;同时,合并感染组与健康对照组相比,肠杆菌、肠球菌数量显著增加,乳酸杆菌、双歧杆菌数量显著减少,且差异具有统计学意义（P<0.05）。结论 单纯患慢性乙肝的患者组和单纯感染华支睾吸虫的患者组存在不同程度的肠道菌群失调,当慢性乙肝患者合并感染华支睾吸虫后,菌群失调状况加剧,使疾病的进程进一步恶化,导致肠道菌群的失调和病程的恶化相互作用,对疾病的预防和治疗产生不良影响。

Effect of Clonorchis sinensis infection on the intestinal microflora of patients with chronic hepatitis B

Objective To understand the growth status of intestinal microflora in patients with chronic hepatitis B who co-infected with Clonorchis sinensis, and to explore the relationship between intestinal microflora and co-infection status, so as to provide advice for clinical prevention and treatment. Methods Using the dilution plate colony counting method, we collected the fresh stool samples from four groups, healthy people, patients infected with chronic hepatitis B only, patients infected with Clonorchis sinensis only, and chronic hepatitis B patients co-infected with Clonorchis sinensis, respectively. There were 30 cases of fresh stool samples per group. We counted the four strains of enterobacteria, Enterococcus, Lactobacillus, and bifidobacteria, and compared the changes of the intestinal microflora of the four groups. Finally, we analyzed the effect of the co-infection status on the intestinal microflora of patients. Results Compared with the healthy control group, the Enterobacter and Enterococcus increased, and lactobacillus and bifidobacterium decreased in patients with chronic hepatitis B only and in patients with Clonorchis sinensis only in varying degrees (P<; 0.05); this phenomenon was more significant for chronic hepatitis B patients co-infected with Clonorchis sinensis (P<; 0.05). Conclusions Intestinal microflora balance exist in different levels in group of patients with either chronic hepatitis B only or Clonorchis sinensis only. Besides, when the patients with chronic hepatitis B are further infected with Clonorchis sinensis, the condition of the intestinal microflora imbalance will be worse. Conversely, the imbalance of intestinal microflora will promote the progression of disease, thus forming a vicious circle. Obviously, the vicious circle goes against the clinical prevention and treatment.