HPLC测定山银花药材中常春藤皂苷元的含量

目的建立山银花药材中的常春藤皂苷元含量的测定方法。方法以盐酸一乙醇溶液为水解溶剂，山银花样品加热回流4h，再用三氯甲烷萃取；采用HPLC，以Waters Symmetry C18（150min×4．6mm，5μm）色谱柱，甲醇-0．4％冰醋酸水溶液（80：20）为流动相，流速为1．0mL／min，柱温为30℃，检测波长为210nm，测定常春藤皂苷元含量。结果山银花中常春藤皂苷元的线性范围为2-12μg，平均加样回收率99．O％，RSD为1．9％。不同产地山银花的常春藤皂苷元含量差异较大．湖南隆回野生的含量高达2＋8％，而云南、贵州产的未检出。结论所建立方法操作简便、准确、可靠、重复性好．可用于山银花药材中常春藤皂苷元的含量测定。

HPLC Determination of Hederagenin in Flos Lonicerae Confusae

Objective To establish a new method for the determination of hederagenin in Flos Lonicerae Confusae. Methods The herb was hydrolyzed with hydrochloric acid-ethanol for 4 hours at 80 ℃ and the hederagenin was extracted with chloroform and analyzed by HPLC. The Waters Symmetry-C18 （150 mm× 4.6 mm,5 μm）was used with the mobile phase of methanol-0. 4% glacial acetic acid （80：20）, the flow rate was 1.0 mL/min, the column temperature was 30 ℃, the detected wavelength was 210 nm. Results The linear range of hederagenin was 2-12μg, the recovery rate was 99.0%, RSD was 1.9%. The content of hederagenin in Flos Lonicerae Confusae was varied with growing areas. The content of hederagenin of wild species from Longhui of Hunan province is up to 2.8%, while the hederagenin of the species from Yunnan and Guizhou province has not been detected. Conclusion The developed method is convenient, precision and repeatability, which can be used to determine the content of hederagenin in Flos Lonicerae Confusae.