TiO2纳米光催化剂薄膜对牛晶状体上皮细胞黏附及移行的影响

目的观察经光激发后TiO2纳米光催化剂薄膜对体外培养的牛晶状体上皮细胞（LECs）黏附及移行的影响。方法采用溶胶-凝胶法在载玻片表面制备TiO2光催化剂薄膜,对载玻片行接触角测量。体外培养牛LECs爬片生长于此薄膜上。未经TiO2纳米光催化剂薄膜修饰的载玻片设为对照。进行细胞黏附和迁移实验,测定并比较牛LECs在TiO2薄膜修饰载玻片与对照载玻片表面的生长、移行情况。结果对TiO2薄膜组和对照组载玻片行接触角测量：对照组载玻片接触角为18.825°±2.342°,TiO2镀膜组载玻片接触角为0°±2°,TiO2镀膜组载玻片的接触角明显小于对照组。通过细胞黏附实验,TiO2镀膜组载玻片表面细胞黏附数平均为36.60±11.20,对照组细胞黏附数平均为96.40±19.67,差异有统计学意义（P=0.000）。细胞迁移实验（划痕法）中,对照组细胞移行距离平均为0.951mm,TiO2镀膜组细胞平均移行距离为0.640mm,差异有统计学意义（P=0.000）。结论TiO2纳米光催化剂薄膜具有超亲水性,能够明显抑制牛LECs在其表面的黏附和移行。

The effects of TiO_2 nano thin film on the migration and adherence of BLEC in vitro

Objective The generate of posterior capsular opacification(PCO)is associated with the adhensive and migration of residual subcapsular lens epithelial cells(LECs).Titanium dioxide(TiO_2)nanometer is proved to have the ability of killing tumor cells and cultured bovine LECs.This study tried to observe the effects of TiO_2 nanometer thin film provoked by light on adhesiveness and migration of bovine LECs in vitro.MethodsThe fresh bovine lenses were obtained and cultured in DMEM containing 10% of newborn bovine serum.The second to fifth generation of cells were used in this experiment.The slide modified by TiO_2 photocatalyst film was prepared by sol-gel method.Cultured cells were seeded in filmed or unfilmed slides respectively and exposed to ultraviolet(wavelength 365 nm)for 20 or 40 minutes.The contact angle between water drop and slide was measured by droping method and the cells adhered to slides were calculated after 24 and 48 hours of culture.The growth status and migration distance of bovine LECs were assayed and compared between filmed and unfilmed groups.ResultsThe contact angle between water drop and slide was 0°±2°and 18.825°±2.342° in filmed and unfilmed group respectively,indicating a obviously smaller contact angle in TiO_2 filmed group than unfilmed one.The numbers of bovine LECs adhered to filmed slide was considerably reduced in TiO_2 filmed group compared with unfilmed group in different UVA exposure time(t_(0 min)=5.492,P=0.001;t_(20 min)=6.031,P=0.000;t_(40 min)=6.828,P=0.000).However,no significant difference was found in the numbers of adhensive cells among 3 UVA irradiation time points(F=1.278,P=0.297).The migration distance of the cells was significantly shorter in TiO_2 filmed group in comparison with unfilmed group in 24 and 48 hours after UVA irradiation(F_(group)=14.965,P=0.000;F_(time)=38.033,P=0.000).ConclusionThe TiO_2 nanometer thin film is characterized by the superhydrophilic property.So it can effectively impede the adhesion and migration of bovine LECs in vitro.