| 四环素对退变腰椎间盘影响的实验研究 |
| |
| 引用本文： | 陈德胜,李燕,杨文君,金群华.四环素对退变腰椎间盘影响的实验研究[J].宁夏医学杂志,2010,32(4):299-301,F0002. |
| |
| 作者姓名： | 陈德胜 李燕 杨文君 金群华 |
| |
| 作者单位： | 1. 宁夏医科大学附属医院,宁夏,银川,750004
2. 宁夏医科大学,宁夏,银川,750004 |
| |
| 摘 要： | 目的观察四环素延缓和抑制大鼠腰椎间盘退变病理变化的作用及与基质金属蛋白酶（MMP）的关系。方法建立大鼠腰椎间盘退变模型。实验设正常组、模型组、四环素治疗组（造模后4周,每日皮下注射四环素25mg,持续2周）。大鼠在退变模型建立8周后处死,HE染色光镜观察各组腰椎间盘组织病理改变,免疫组织化学和蛋白印记实验检测腰椎间盘组织MMP-3的表达。结果动物模型建立8周后除正常组外,其它各组椎间盘组织均出现不同程度退行性病理改变,四环素治疗组病理改变较模型组减轻。四环素治疗组椎间盘组织中MMP-蛋白表达呈弱阳性,与模型组差异有统计学意义（P〈0.05）,与正常组无统计学意义。蛋白印记检测同免疫组化结果相同。结论MMP-3的增高可导致椎间盘细胞外基质降解,而四环素能降低MMP-3的表达,从而减轻椎间盘基质的降解,延缓和抑制实验性大鼠腰椎间盘退变的形成。
|
| 关 键 词： | 四环素 大鼠 腰椎 椎间盘退变 |
Research on the impacts of tetracycline in rat lumbar degeneration intervertebral disc |
| |
| CHEN De-sheng,et al..Research on the impacts of tetracycline in rat lumbar degeneration intervertebral disc[J].Ningxia Medical Journal,2010,32(4):299-301,F0002. |
| |
| Authors: | CHEN De-sheng |
| |
| Institution: | The Affiliated Hospital of Ningxia Medical University/a>;Yinchuan 750004/a>;China |
| |
| Abstract: | Objective To observe the change of rat lumbar degeneration intervertebral disc and the relationship between degeneration and MMP3.Methods The rats were divided into normal control group,degenerative control group and experimental group(16 rats in group).The rats in experimental group were treated by tetracycline(25mg/day,subcutaneously) for two weeks.Rat lumbar discs were examined by light microscopy.The expression of MMP-3 was observed in disc tissue by using immunohistochemistry and Western Blot.Results L... |
| |
| Keywords: | Tetracycline Rat Lumbar Degeneration interverbral disc |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
