水飞蓟素对大鼠缺血再灌注损伤的保护作用

目的　探讨水飞蓟素对大鼠缺血再灌注损伤的保护作用。方法　 2 0只大鼠随机分为对照组和观察组 ,各 10只 ,两组术前分别灌服 0 .5 %羧甲基纤维素钠 (CMCNa)溶液和水飞蓟素CMCNa混悬液 ,观察组大鼠腹主动脉缺血 30 m in后再灌注 4 5 m in,检测并比较两组血清中丙二醛 (MDA )及超氧化物歧化酶 (SOD)的变化。结果　两组缺血前血清中 MDA含量及 SOD活性无显著差异 (P>0 .0 5 )。再灌 4 5 min后 ,两组 MDA含量均有升高 ,但观察组明显低于对照组 (P<0 .0 1) ;再灌 4 5 m in后 ,血清中 SOD均有所下降 ,但观察组下降幅度明显低于对照组 (P<0 .0 1)。结论　水飞蓟素对缺血再灌注损伤具有保护作用。

Protective effects of Silymarin on ischemia-reperfusion injury in rats

Objective To investigate the protective effects of Silymarin on ischemia-reperfusion injury in rats. Methods Sprague Dawley(SD) rats were randomly divided into 2 groups including one control group(n=10) and the observation group(n=10) . The two groups were separately given 0.5% carboxymethylcellulose Na(CMCNa)solution and CMCNa suspension of silymarin , then the observation group was treated with 30 min ischemia followed by 45 min reperfusion. Serum levels of malondialdehyde(MDA)and superoxide dismutase(SOD) after being treated with Silymarin were detected and compared.Results The MDA and SOD were not obviously different in both serum before ischemia(P>0.05),but after 45 min reperfusion , MDA content significantly increased and the observation group was obviously lower than the control one (P<0.01),while SOD activities declined and the observation group was obviously higer than the control one (P<0.01). Conclusion Silymarin can protect tissue against ischemia-reperfusion injury.