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One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-gel blue chromatography |
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| Authors: | C. Bruck D. Portetelle C. Glineur A. Bollen |
| Affiliation: | 1. Laboratoire de Chimie Biologique Rhode-St-Genèse, Belgium;2. Laboratoire de Génétique, Université Libre de Bruxelles, Départment de Biologie Moléculaire, 67 rue des Chevaux, B-1640 Rhode-St-Genése, Belgium |
| Abstract: | Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities. |
| Keywords: | Affi-gel blue chromatography monoclonal antibody purification PBS phosphate-buffer saline BSA bovine serum albumin SPRIA solid-phase radioimmunoassay SDS-PAGE sodium dodecyl sulfate-polyacrylamide gel electrophoresis DEAE diethylaminoethyl |
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