抑郁及糖尿病对大鼠心室肌离子通道表达的影响

目的:探讨抑郁及糖尿病对大鼠心室肌离子通道表达的影响。方法:将雄性SD大鼠随机分为健康对照组、抑郁组、糖尿病组及抑郁合并糖尿病组。抑郁动物模型通过持续4周的慢性温和应激获得。糖尿病动物模型通过皮下注射四氧嘧啶获得。4周后,分别取各大鼠左室心肌,采用实时荧光定量PCR方法对其L型钙通道、钠钙交换体(NCX)、瞬时外向钾通道的表达进行定量分析。结果:与健康对照组比较,抑郁组L型钙通道和NCX的表达增加,糖尿病组瞬时外向钾通道的表达减少,抑郁合并糖尿病组NCX的表达增加、瞬时外向钾通道的表达减少;抑郁合并糖尿病组瞬时外向钾通道的表达较抑郁组减少;抑郁合并糖尿病组NCX的表达较糖尿病组增加。结论:抑郁及糖尿病可引起大鼠心室肌离子通道表达的改变。抑郁与糖尿病对大鼠心室肌离子通道表达的影响有一定的累积作用。

The effects of depression and diabetes mellituson expression of ventricular ion channel in rats

Objective：To determine the effects of depression and diabetes mellitus on expression of ventricular ion channel in rats. Methods：Male Sprague-Dawley rats were randomly divided into health control group, depression group, diabetes group and depression with diabetes group. The model of depression was estublished by chronic mild stress for 4 weeks. The model of diabetes was estublished by injecting subcutaneouly alloxan. After 4 weeks, the expression levels of L-type Ca^2＋ channel, Na^＋/Ca^0＋ exchanger （NCX）, transient outward K^＋ channel in the myocardium of the left ventricular in each rat were examined by quantitative real time RT-PCR. Results ：The expression levels of L-type Ca^2＋ channel and NCX were higher in depression group than those in health control group. The expression level of transient outward K^＋ channel was lower in diabetes group than that in health control group. Compared with health control group, depression with diabetes group had increased expression of NCX,decreased expression of transient outward K^＋ channel. Compared with depression group ,depression with diabetes group had decreased expression of transient outward K^＋ channel. Compared with diabetes group, depression with diabetes group had increased expression of NCX. Conclusion：Depression and diabetes may change expression of ventricular ion channel in rats. Depression and diabetes may have accumulated effects on expression of ventricular ion channel in rats.