内皮型一氧化氮合酶基因G894T多态性和急性冠脉综合征的关系

目的:探讨内皮型一氧化氮合酶(eNOS)基因位点G894T多态性与急性冠脉综合征(ACS)的关系.方法:采用聚合酶链反应一限制性片断长度多态性(PCR-RFLP)方法.对260例ACS患者(急性心肌梗死120例,不稳定型心绞痛140例)和248例同期住院且都经冠脉造影排除冠状动脉粥样硬化性心脏病(CHD)的患者,进行eNOS基因位点G894T多态性的分析,比较各基因型及等位基因的频率在两组患者中差别,并分析其与不同血管病变支数之间的关系.结果:GT TT基因型频率及T等位基因频率在ACS组(23.46%,14.42%)显著高于对照组(9.27%,5.24%)(P＜0.01).GT TT基因型频率及T等位基因频率在ACS亚组急性心肌梗死(AMI)组和不稳定型心绞痛(UAP)组之间无统计学差异,且与血管病变支数无关(P＞0.05).结论:eNOS基因G894T多态性的T等位基因可能是ACS的重要遗传危险因素,但与ACS不同临床类型(AMI和UAP)及血管病变的严重程度无关.

Relationship between G894T polymorphism of endothelial nitric oxide synthase gene and acute coronary syndrome

Objective:To assess the effect of G894T polymorphism in exon 7 of the endothelial nitric oxide synthase gene(eNOS) on the development of acute coronary syndromes(ACS).Methods:By means of Polymerase Chain Reactiun and Restriction Fragment Length Polymorphism,G894T Polymorphism of eNOS was detected in 260 patients with ACS(120 with acute M1 and 140 with unstable angina)and 248 subjects excluded CHD by coronary angiography.Results:Genotypes GT TT and T allele frequency in ACS group were significant higher than those in control group(23.46% vs 9.27%,14.42% vs 5.24%)(P<0.01).There was no significant difference in genotypes GT TT between AMI subgroup and UAP subgroup(20.83% vs 25.71%,P>0.05).That did not correlate with the number of coronary vessels diseased in patients with ACS.Conclusion:The T allele of eNOS gene might be associated with ACS and a risk genetie marker of CHD evolution in ACS.But that is not related to the severity of coronary diseased.