成年大鼠脑出血后室管膜下区、血肿周围神经前体细胞增殖研究

目的 研究成年大鼠脑出血后室管膜下区、血肿周围组织神经前体细胞增殖的规律.方法 立体定向注射胶原酶建立大鼠纹状体脑出血模型.脉冲法或连续法腹腔注射Brdu标记增殖细胞.在脑出血后第2、7、14及28天处死大鼠,行Brdu免疫组化染色.对室管膜下区、血肿周围的Brdu免疫阳性细胞作细胞记数.结果 脉冲法Brdu标记.大鼠脑出血后第2天双侧室管膜下区和血肿周围Brdu阳性细胞数增加,第7天时达高峰,第14天仍可见较多的增殖细胞;连续法Brdu标记.在脑出血后第14天时室管膜下区和血肿周围可见较多Brdu阳性细胞,第28天时室管膜下区Brdu阳性细胞数降至对照水平.但血肿周围仍可见较多的Brdu阳性细胞.结论 成年大鼠脑出血后可诱导双侧室管膜下区和血肿周围神经前体细胞增殖增加;室管膜下区增殖细胞可能向血肿周围迁移.

Proliferation of neural progenitor cells in the subventricular zone and around the hematoma after intracerebral hemorrhage in adult rat

Objective To examine the proliferation of the neural progenitor cells in the subventricular zone (SVZ) and around the hematoma after intracerebral hemorrhage (ICH) in adult rats.Methods ICH was induced by stereotactic injection of type VII collagenase into the corpus striatum of adult rats,followed by pulse or continuous intrapenitoneal injection of bromodeoxyuridine (Brdu) to label the proliferating cells.The rats were sacrificed on days 2,7,14 and 28 following the ICH for immunohistochemistry of the tissu...