Combined effect of tumour necrosis factor-α and interleukin-13 polymorphisms on bronchial hyperresponsiveness in Korean children with asthma |
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Authors: | H-B. Kim M-J. Kang S-Y. Lee H-S. Jin J-H. Kim B-S. Kim S-O. Jang Y-C. Lee M-H. Sohn K-E. Kim S-J. Hong |
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Affiliation: | Department of Pediatrics, Inje University Sanggye Paik Hospital, Seoul, Korea,;Asan Institute for Life Sciences, University of Ulsan College of Medicine, Seoul, Korea,;Department of Pediatrics, Hangang Sacred Heart Hospital, Hallym University College of Medicine, Seoul, Korea,;Department of Pediatrics, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea,;Department of Internal Medicine and Research Center for Allergic Immune Diseases, Chonbuk National University Medical School, Jeonju, Korea, and;Department of Pediatrics and Institute of Allergy, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Korea |
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Abstract: | Background TNF‐α and IL‐13, two pivotal pro‐inflammatory cytokines, are increased in asthmatic airways and may be linked to asthma susceptibility and/or bronchial hyperresponsiveness (BHR). Objective We investigated the association between the TNF‐α?308G/A polymorphism and asthma susceptibility or asthma‐related phenotypes in Korean children with asthma, and tested for a combined effect with IL‐13 polymorphisms. Methods Asthmatic children (n=719) and non‐atopic healthy control children (n=243) were evaluated for asthma phenotypes including total serum IgE and BHR to methacholine. Genotypes were determined by PCR‐restriction fragment length polymorphism analysis. Results The allele frequency of TNF‐α?308A in asthmatics (14.1%) was higher than that in control children [8.7%, odds ratio (OR) 1.72, 95% confidence interval (CI) 1.05–2.82]. Significantly lower PC20 values were found in asthmatic children carrying one or two copies of the TNF‐α risk allele (?308A) vs. those homozygous for the common allele (P=0.026). Combined analysis revealed that atopic asthmatic children co‐inherited the risk alleles of TNF‐α?308G/A and IL‐13 +2044G/A more frequently than control children (aOR 1.91, 95% CI 1.00–3.65), and asthmatic children co‐inheriting both risk alleles had significantly lower PC20 values vs. asthmatic children homozygous for the common alleles (P=0.024). Conclusion The TNF‐α promoter polymorphism (?308G/A) may be associated with asthma susceptibility and BHR in Korean children with asthma. In addition, there appears to be a synergistic effect between the TNF‐α promoter polymorphism and an IL‐13 coding region polymorphism in terms of asthma susceptibility and BHR in this population. |
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Keywords: | asthma bronchial hyperresponsiveness combined effect IL-13 TNF-α |
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