聚合酶链反应对胆囊癌组织中细菌DNA片段的检测研究

目的　研究胆囊癌组织及相应胆汁中的细菌菌谱，以探讨胆囊癌的发生与细菌感染的关系。方法　采用聚合酶链反应（ＰＣＲ） ，对３７ 例胆囊癌组织标本及９ 例相应胆汁中的细菌ＤＮＡ 片段进行分子生物学扩增。结果　３７ 例胆囊癌组织标本中细菌ＤＮＡ 片段检出率为７８－３７％ （２９／３７），９ 例相应胆汁中细菌ＤＮＡ检出率为７７－８％ （７／９）。结论　胆囊粘膜癌变可能与厌氧菌尤其是产气荚膜梭菌感染存在联系，厌氧菌与需氧菌协同作用，长期刺激胆囊粘膜可能是引起癌变的主要因素。

Detection of bacterial DNA in gallbladder carcinoma tissue by polymerase chain reaction

Objective To find out the relation between bacterial infection and formation of gallbladder carcinoma. Method By using polymerase chain reaction, we detected bacterial DNA in gallbladder carcinoma tissue of 37 patients. Nine gallbladder carcinoma patients' bile was also detected by PCR. Results The positive rate of bacterial DNA in bile was 77.8%(7/9) and in cancerous tissue was 78.37%(29/37). Conclusion There is certain relation between gallbladder carcinoma and infection of anaerobic bacteria, espacially of C. Perfringers. Stimulaton of gallbladder mucosa by anaerobic and aerobic bacteria together might be the main cause leading to formation of carcinoma.