细胞周期相关基因的表达与壶腹癌细胞增殖关系的研究

目的探讨细胞周期调控相关基因ｐ５３、ｐ２１、ｐ１６和Ｒｂ与壶腹癌细胞增殖之间的关系及细胞增殖相关蛋白Ｋｉ－６７与壶腹癌生物学特性和预后之间的关系。方法采用免疫组化ＡＢＣ法对４０例壶腹癌和１０例正常壶腹组织进行染色。结果４种抑癌基因在壶腹癌中的阳性表达率分别为５０％、６２．５％、４７．５％和９２．５％。ｐ２１、ｐ１６的表达与Ｋｉ－６７指数呈负相关（Ｐ＜０．０５），ｐ５３、Ｒｂ与Ｋｉ－６７指数无相关性（Ｐ＞０．０５），而Ｋｉ－６７指数与壶腹癌的病理分级、临床分期明显相关（Ｐ＜０．０５）。结论ｐ２１，ｐ１６异常表达与壶腹癌细胞异常增殖有关。Ｋｉ－６７指数可能有助于了解壶腹癌的生物学特性并对预后进行判断。

Study of the relationship between expression of cell cycle-related genes and proliferation of ampullary carci-noma cells

Objective To study the relationship between the cell cycle-related genes p53, p21, p16, Rband proliferation of ampullary carcinoma cells, and relationship between the cell proliferation-related Ki-67 proteinand biological property and prognosis of ampullary carcinam. Method The expression of these 4 genes and Ki-67protein in tissues of normal ampullas (n=10) and of ampullary carcinoma(n=40) was detected by immunohistochemical ABC method. Results The positive rates of p53, p21, p16 and Rb proteins in ampullary carcinomawere 50%, 62.5%, 47.5% and 92.5%, respectively. The correlation between the expression of p21, p16 proteins and Ki-67 index wes negative. No association o f p53, Rb expressions with Ki-67 index wes found. There wereclose relationships between the Ki-67 index and histological grades, clinical stages. Conclusion Abnormal expession of p21, p16 proteins is associated with tumor cell proliferation and the detection of Ki-67 index may beuseful in understanding biological characteristics as well as evaluating prognosis in ampullary carcinoma.