核仁组成区相关蛋白在原发性胆囊癌诊断中的评价

应用检测核仁组成区相关嗜银蛋白(AgNOR)染色方法,对15例胆囊粘膜上皮单纯增生、12例不典型增生以及46例原发性胆囊癌进行纳胞学研究。从中发现细胞核中AgNOR颗粒的多寡及大小,对确定不典型增生和癌具有重要意义。不典型增生组和癌症组每个细胞核内AgNOR颗粒大小及平均数明显高于上皮增生组(P<0.01)。研究结果表明,检测核仁区AgNOR颗粒,可使胆囊粘膜上皮不典型增生和癌诊断的判定更为客观。我们认为AgNOR技术作为组织病理学的辅助诊断技术,应更广泛地运用于肿瘤研究中。

Evaluation of Nucleolar Organizer Region-associated Proteins in Primary Carcinoma of the Gallbladder Mucous Epithelium

Using a. silver staining technique, nucleolar organizer region-associated proteins (AgNOR,) Have been studied in paraffin section of 15 simple hyperplasia, 12 atypical hyperplasia and 46 primary carcinoma of the gallbladder mucous epithelium. The results showed that the average number and size of AgNOR per nucleus were of important significance in defining atypical hyperplasia and carcinoma. It was found that the number and size of AgNOR per nucleus in malignant gallbladder lesions significantly exceeded those of benign lesions. The results indicate that the average number and size of AgNOR per nucleus can be used to define the degree of atypical hyperplasia and carcinoma in the epithelium of gallbladder mucous more objectively, It is suggested that the AgNOR technique can be applied incresing as an adjunct to diagnostic histopathology.