补肾活血中药对免疫介导再障小鼠骨髓CD45+细胞的影响

目的:观察补肾活血中药对免疫介导再障小鼠CD₄₅⁺细胞的影响。方法:建立免疫介导再障小鼠模型,随机分组胃饲生理盐水、高剂量中药、低剂量中药、环孢素A(CsA),并设正常对照,第10d分别称体重、计算外周血细胞和骨髓有核细胞数、采用流式细胞技术测定骨髓CD₄₅⁺细胞。结果:中药组小鼠体重、外周血细胞、骨髓有核细胞数、骨髓CD₄₅⁺细胞显著高于空白模型组和CsA组,而接近正常对照组。结论:补肾活血中药对免疫介导再障小鼠的骨髓造血细胞有保护作用。

Study on efficacy of the decoction of invigorating kidney and activating blood circulation in BALB/C mice with immune mediated aplastic anemia

AIM and METHODS: To study the efficacy of the decoction of invigorating kidney and activating blood circulation(DIKABC) in BALB/C mice with immune mediated aplastic anemia. 50 female BALB/C mice at 8~12 weeks of age were divided into 5 groups:(1) control,(2) model,(3) model+DIKABC(H), (4) model+DIKABC(L), and (5) model+ciclosporin A(CsA). The model of immune mediated aplastic anemia in BALB/C mice, which received sublethal whole body irradiation, lymph node and thymus cells of DBA/2 mice, was given different liquid from groups and administered appropriately for 9 days, blood cells and bone marrow nucleated cells(BMNC) and CD₄₅⁺ cells were determined by flow cytomytry(FCM).RESULTS: RBC,WBC,Platelet,BMNC in DIKABC groups were higher than those in model group (P＜0.01/0.05), but those in CsA group were lower than those in DIKABC groups (P＜0.05).Compared with model group, CD₄₅⁺ cells in DIKABC groups increased (P＜0.05),but were lower than those in control groups (P＜0.05). CONCLUSION: The decoction of invigorating kidney and activating blood circulation could increase the percentages of CD₄₅⁺ cells in bone marrow and prevent immune mediated aplastic anemia in BALB/C mice.