广州市严重急性呼吸综合征患者血清IgG抗体滴度变化规律和隐性感染情况调查

目的　了解严重呼吸道综合征 (SARS)患者血清中特异性IgG抗体滴度变化规律和健康人群感染SARS冠状病毒的状况 ,并为临床诊断和血清流行病学调查提供可靠依据。方法　 2 0 0 3年 5～ 6月份选择发病 1、 2、 3、 4、 5月的SARS患者 ,及无临床症状的健康人群作为调查对象 ,用间接ELISA法检测SARS冠状病毒IgG抗体。 结果　对 5 33份发病后 14～ 15 3d的SARS患者的血清进行IgG抗体检测 ,其中 2 6 5例阳性。发病 1个月IgG抗体GMT为 5 4 96 ,发病 2个月为 12 2 5 6 ,发病3个月为 135 2 5 ,发病 4个月为 15 2 17,发病 5个月为 183 79。SARS患者血清IgG抗体随着发病时间的延长而呈上升趋势。发病 1个月与 2、 3、 4、 5个月抗体水平相比差异有统计学意义 (P <0 0 1) ,发病 2、 3、 4、 5个月抗体水平相比差异无统计学意义。对照人群中共 2 16人份 ,仅发现 1例IgG抗体阳性。 结论　SARS发病 1个月IgG抗体水平比较低 ,1个月后迅速上升 ,第 4、 5个月仍持续在较高抗体水平。正常人群隐性感染水平低

Study on Rule of Titre Changes of SARS-serum IgG Antibody in SARS Patients and Latent Infector in Guangzhou

Objective To understand the trend of SARS-serum IgG titre changes in patients with SARS and SARS subclinical infection in normal population in Guangzhou. And to provide reliable bases for clinical diagnosis and serum epidemiological inrestigation. Methods To detect SARS IgG for 533 blood specimens obtained from SARS patients in May-June 2003 and 216 normal blood samples by ELISA assay,and measure the titre of antibody by serial dilution for SARS-IgG-positive samples.Results 265 of 533 blood specimens 14-153 days after infection with SARS and one of 216 normal blood samples were SARS-IgG positive.The geometrical mean titres(GMT) of SARS-IgG were 54.96,122.56,135.25,152.17 and 183.79 respectively in patients with SARS for 1,2,3,4 and 5 months .The difference was found between SARS patients with one month infection and those with 2,3,4,5 months infection by tendency F test(P<0.01),but there was no difference among those infected SARS for more than one month.Conclusions The titre of SARS-IgG was low in patients with SARS for one month, but increased quickly the after first month still persisted in high level in the 4th and 5th month. In addition,there might be low subclincal infection with SARS coronavirus in general population.