黄芪总苷对肝星状细胞增殖和合成胶原的抑制作用

目的　探讨黄芪总苷 (AST)对肝星状细胞增殖和合成胶原的影响。方法　采用枯细胞条件培养基 (KCCM )及含新生牛血清 (NBS)和健康大鼠血清 (RS)的混合血清刺激大鼠肝星状细胞 (HSC)系HSC T6 ,用 3H TdR和3H 脯氨酸参入法检测HSC增殖活性和胶原合成状况。结果　AST(1 6、32、64、1 2 8和 2 56mg·L- 1 )对KCCM 1∶4刺激HSC T6细胞增殖和胶原合成均有明显的抑制作用 ;在含 1 0 %NBS- 3 %RS混合血清刺激的HSC T6细胞 ,AST(32、64、1 2 8和 2 56mg·L- 1 )作用 48h对HSC T6细胞增殖 ,及AST(1 6、32、64、1 2 8和 2 56mg·L- 1 )作用 72h对HSC T6细胞胶原合成均有明显的抑制作用 ,呈浓度依赖型趋势。结论　AST对体外激活肝星状细胞的增殖和产生胶原有明显抑制作用 ,该作用可能是AST抗肝纤维化的机制之一

Effect of Astragalosides on proliferation and collagen production of hepatic stellate cells in vitro

AIM To study the effect of astragalosides (AST) on pr ol iferation and collagen production of hepatic stellate cells (HSC). METHO DS The proliferation and collagen production of rat hepatic stellate cell s, HSC-T6, stimulated with Kupffer cell-conditioned medium (KCCM), mixed sera containing new bovine serum (NBS) and rat serum (RS) were measured with 3H -TdR and 3H-proline incorporation. RESULTS Both of the p roliferation and collagen production of HSC-T6 stimulated with KCCM at the dilu tion of 1∶4 were suppressed after being treated with AST (16, 32, 64, 128 and 2 56 mg·L -1 ). When HSC-T6 cells were stimulated with mixed sera containing 10% NBS and 3% RS, their proliferation was suppressed after being treated with AST(32, 64, 128 and 256 mg·L -1 )for 48 h and so was the collagen produ ction after being treated with AST(16, 32, 64, 128 and 256 mg·L -1 )for 72 h. CONCLUSION The suppression of hepatic stellate cell prolif eration and collagen production by astragalosides may be one of the mechanisms f or the depression of hepatic fibrosis by astragalosides.