急性胰腺炎大鼠模型的制备和研究

目的 学习急性胰腺炎大鼠模型的复制及研究方法，对探讨急性胰腺炎的发病机制，从而为临床对急性胰腺炎的诊断、治疗及预防提供实验依据。方法 根据胆汁返流学说，经胰胆管向大鼠胰腺组织内逆行注射4％去氧胆本钠，动态检测血清淀粉酶活性的及各组织形态学的变化。结果 实验组大鼠胰腺出现明显充血、水肿及片状坏死，有大量白细胞浸润，肝脏、肺、肾、回肠等组织形态学也随病程加重而有不同程度的异常，血清淀粉酶活性明显升高。结论 去氧胆酸钠通过激活胰蛋白酶原，引起胰腺的自身消化性损害，从而产生急性胰腺炎的临床表现和病理改变。

A Study on Acute Pancreatitis Model of SD Rats

Objective To find out a simple method to establish an acute pancreatitis model of SD rats, and to inquire into the pathogenesis of the acute pancreatitis(AP) in SD-rats, to provide experimental evidences for diagnosis, treatment and prevention of the disease.Methods Twenty SD-rats were randomly assigned into two groups: normal control group(n=4) and AP model group(n=16) which was induced by injecting 4% sodium deoxycholate(NaTc) (0.1 ml/100 g) into the pancreatic-bile duct system in rats. The serum amylase levels were tested. The morphology of several organs was observed at the time of 1h and 6h after induction, respectively.Results The pancreas of the AP group appeared cogestion, edematous and massive necrosis, infiltrated with an abundance of leukocytes. The morphology of liver, lungs, kidney, ileum also showed abnormality in different degrees with the aggravation of the course of the disease.Conclusion NaTc can cause acute pancreatic auto-digestion damage through acting pancreatic trypsinogen, thus create the clinical manifestation and the pathological change of acute pancreatitis.