丙型肝炎病毒包膜蛋白E2抗原模拟表位的筛选和鉴定

目的：筛选丙肝病毒（HCV）包膜蛋白（E2）特异性噬菌体模拟表位。方法：以抗－HCV E2的单克隆抗体作为固相筛选分子，对噬菌体同七肽库进行5轮“吸附－洗脱－扩增“的筛选过程，随机挑取50个克隆，经酶联免疫吸附法（ELISA）鉴定、交叉反应以及竞争抑制性实验，最后对所选克隆进行DNA序列分析，以确定HCV E2抗原的模拟表位。结果：经噬菌体富集后，得到12个阳性克隆，确定氨基酸序列XXPXYXW为HCV E2的模拟表位。结论：用噬菌体七肽库成功筛选得到HCV E2的模拟表位，为开展用HCV模拟表位探索HCV的防治研究创造了条件。

Epitope mapping of hepatitis C virus E2 antigen from a 7 peptide phage library by using immobilized specific monoclonal antibody

Objective:To screen HCV E2 mimotopes by using monoclonal antibody and phage peptide library.Methods:By using HCV E2 monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. Results:12 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparision results, one epitope was confirmed as mimotope of HCV E2. Conclusion: HCV E2 mimotope was obtained by phage peptide library screening. The result provides a new approach for HCV therapy and vaccine development.