结直肠腺癌发病基因fbxl20和E-cadherin的相关性研究

目的研究fbxl20与E—cadherin基因与结直肠癌临床病理特征的关系及其表达水平间的相关性。方法收集50例结直肠腺癌组织及对照癌旁组织，用半定量PCR技术检测fbxl20及E—cad．herin基因的表达水平并进行相关性分析。结果fbxl20基因在结直肠腺癌组织中的表达水平显著高于癌旁组织（0．479±0．141 vs．0．296±0．121，P=0．001）；E—cadherin基因在结直肠腺癌组织中的表达水平显著低于癌旁组织（0．440±0．026vs．0．741±0．059，P=0．000）；fbxl20与E—cadherin基因在本研究50例结直肠腺癌组织中的表达水平无相关性（r=-0．165，P=0．251）。但是，在低分化和高分化肿瘤组织及D期肿瘤组织中，fbxl20与E—cadherin基因的表达水平呈负相关（r=-0．600，P=0．008；r=-0．784，P=0．017；r=-0．643，P=0．032）。结论E—cadherin基因在结直肠腺癌组织中的低表达可能与fbxl20基因的高表达有关，且结直肠腺癌晚期肿瘤的高迁移性及高侵袭性可能与E—cadherin有关。

The research about the correlation of colorectal adenocarcinoma gene fbxl 20 and E - cadherin

Objective To study the relationship between the fbxl 20 gene, E - cadherin gene and the clinocopathologic features,respectively, and the correlation about the fbxl 20 and the set gene. Methods The mRNA expression level of fbxl 20 and E - cadherin gene in 50 pairs of human colorectal adenocarcinoma tissues and matched normal tissues were detected by RT - PCR. Results The mRNA expression level of fbxl 20 gene in tumor tissues were significantly increased than that in normal tissues （0. 479 ± 0. 141 vs. O. 296 ± 0. 121, P = 0.001 ）, while the E - cadherin were decreased （ 0. 440 ± 0.026 vs. O. 741 ± 0. 059, P = 0, 000）. There was no found on the correlation between the mRNA expression level of fbxl 20 and the E - cadherin gene in the 50 tumor tissues（r = -0. 165, P = 0.251 ）. However, the E -cadherin expressed level was negatively correlated with the fhxl 20 expressed level in the lower differentiation degree,in the high differentiation degree and the Duke＇s D tumor tissues（r= -0.600,P=0.008;r= -0.784,P=0.017;r= -0.643,P=0.032）.Conclusions The decreased mRNA expression level of E - cadherin in tumor tissues are largely due to the increased fbxl 20 gene expressed level, which is related with the high mobility and invasion ability of the advanced tumor.