硼替佐米联合化疗药物对肝癌、 结肠癌细胞的抑制作用研究

目的探索亚砷酸对肝癌细胞和结肠癌细胞的作用；研究硼替佐米对肿瘤细胞化疗效果的效应，初筛提高化疗效果的硼替佐米与常用化疗药物的最佳组合方案。方法M。rr法检测细胞的增殖情况，确定各药物的IC50（半数抑制浓度）；硼替佐米作用肿瘤细胞系2h、4h、8h后加入化疗药物，进行MTT法、TUNEL法和AnnexinV—PI法检测细胞的增殖和凋亡情况。结果亚砷酸对BEL-7402和HT-29的24h抑制率分别为0．59±0．09、0．71±0．12；氟尿嘧啶、奥沙利铂、亚砷酸（mg／L）作用肝癌细胞BEL-7402的Ic。分别为：5．33±0．07、28．73±O．72、25．93±4．05，而结肠癌细胞HT-29的IC50分别为：7．33±1．13、53．94±1．23、21．93±2．05。联合应用硼替佐米后，肿瘤细胞抑制率及凋亡率提高。结论亚砷酸对肿瘤细胞生长存在明显抑制作用；联合应用硼替佐米与单药抑制率比较，可以显著增强肿瘤细胞对药物的敏感性，提高肿瘤治疗的效果。

Effect of Bortezomib combined with chemotherapeutic medicine on hepatic and colonic cancer cell lines

Objective To explore the influence of arsenous acid on hepatic and colonic cancer cell lines BEL - 7402 and HT - 29. The effect of bortezomib combined with chemotherapeutic medicine and the optimal combination plan of them were also investigated. Methods We detected cell proliferation by MTT to get the inhi- bition rate of two cell lines and further to determine ICs0 （ inhibitory concentration 50% ） of 5 - Fu, oxaliplatin or arsenous acid ;preincubated cancer ceils for 2 h,4 h or 8 h by bortezomib. Chemotheraputie medicine were com- bined respectively. The cell proliferation and apoptosis were analysed by MTr, TUNEL and Annexin V - PI. Re- suits The 24 h inhibition rate of arsenous acid to BEL -7402 and HT -29 was：0.59 ± 0.09,0.71 ±0.12 re- spectively ; the IC50 of 5 - Fu, Oxaliplatin or arsenous acid to BEL - 7402 was ： 5.33 ± 0.07 mg/L, 28.73± 0.72 mg/L,25.93 ± 4.05 mg/L, while to HT - 29 was ： 7.33 ± 1. 13 mg/L,53.94± 1.23 mg/L, 21.93±2.05 mg/L. Both inhibition rate and apoptosis rate were enhanced when chemotheraputic medicine was combined with bortezomib. Condusion Arsenous acid can inhibit the growth of cancer cells obviously ; the susceptibility of each chemotherapy medicine can be strengthened significantly when it is combined with bortezomib ,P 〈 0.05.