| 新生儿柯萨奇B＿3病毒性心肌炎及其病原学检测 |
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| 引用本文： | 叶鸿瑁,王雪梅,何小玲,尹智明,彭大成,宫恩聪,柯碧波,肖俊. 新生儿柯萨奇B＿3病毒性心肌炎及其病原学检测[J]. 北京大学学报(医学版), 1995, 0(4) |
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| 作者姓名： | 叶鸿瑁 王雪梅 何小玲 尹智明 彭大成 宫恩聪 柯碧波 肖俊 |
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| 作者单位： | 北京医科大学第三医院儿科，北京医科大学病理学系，北京医科大学公共卫生学院 |
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| 摘 要： | １９９２年北京市某医院发生一次新生儿柯萨奇Ｂ３病毒感染流行，发病３５例，合并病毒性心肌炎８例，２例死亡，尸解病理诊断为急性弥漫性非化脓性心肌炎。为明确病原学诊断，用ＥＬＩＳＡ测患儿血中柯萨奇Ｂ病毒抗体ＩｇＭ；用ＰＣＲ测患儿血及心肌组织中的肠道病毒（包括柯萨奇病毒）ＲＮＡ；用大便及咽拭子分泌物进行病毒分离，证实此次流行的病原为柯萨奇病毒Ｂ３。认为ＥＬＩＳＡ、ＰＣＲ方法快速、敏感、特异强，可推广应用。
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| 关 键 词： | 柯萨奇病毒B／分离和提纯，心肌炎／微生物学 |
COXSACKIE VIRUS B3 MYOCARDITIS AND ETIOLOGICAL DIAGNOSIS OF VIRAL MYOCARDITIS |
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| Affiliation: | Department of Pediatrics,The Third Hospital Ye Hongmao,et al |
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| Abstract: | An epidemic of coxsackie virus B infection occurred at the nursery of a maternity hospital in Beijing in Octo-ber,1992.Thirty five cases were involved.Eight of Thirty five cases suffered from viral myocarditis.Two cases-died.Their clinical manifestations and etiological diagnosis were discussed.The Enzyme-Linked Immunosorbent assay(ELISA)was Employed to detect coxsackie virus B-specific IgM antibodies.The Polvmerase Chain Reaction(PCR)was used to detect enterovirus RNA(including coxsackie virus).Both ELISA and PCR are specific,easy and convenient assay for etiological diagnosis. |
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| Keywords: | Headings Coxsackie B Viruses/isol Myocarditis/microbiol |
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