盾叶薯蓣根状茎不同部位和不同生长期薯蓣皂苷元含量的差异性研究

目的 揭示盾叶薯蓣根状茎不同部位和不同生长期中皂苷的积累与分布以及薯蓣皂苷元含量的差异。方法组织化学和高效液相色谱法(HPLC)。结果 皂苷主要分布在基本组织内，有小维管束分布的区域皂苷积累最丰富，薯蓣皂苷元的含量也最高；其次是无维管束分布的区域：有大维管束分布的区域皂苷积累最少，薯蓣皂苷元的含量最低。结论 雄株3个区域的皂苷元含量均明显的高于雌株，雌雄同株其含量界于雄株和雌株之间。

Studies on diversity of diosgenin in different part of rhizome of Dioscorea zingiberensis in various growing period

ObjectTo study the accumulation and distribution of dioscin in the different part of rhizome of Dioscorea zingiberensis C. H. Wright and the differences of its diosgenin. Methods Histochemistry has been used to study the accumulation and distribution of dioscin in the different part of rhizome and diosgenin was determined by HPLC. Results Dioscin distributed mainly in ground tissue. The amount of dioscin accumulated in the plant part from the region distributed with small vascular bundle (SVB) is the most abundant, and the diosgenin is also the highest in the same region; both of them in the region with no vascular bundle (NVB) are next to that of SVB; the amount of dioscin accumulated in the region distributed with big vascular bundle (BVB) is the lowest, and the diosgenin is the lowest in the same region too. Conclusion Diosgenin in every region of the three of male rhizome is higher than that of female rhizome and diosgenin in every region of the three of monoecism is between those of male and female rhizome.