| 超声波协同复合酶法提取半边莲多糖工艺优选 |
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| 引用本文： | 黄秀香,赖红芳,罗丽程.超声波协同复合酶法提取半边莲多糖工艺优选[J].中国实验方剂学杂志,2012,18(5):44-46. |
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| 作者姓名： | 黄秀香 赖红芳 罗丽程 |
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| 作者单位： | 河池学院化学与生命科学系,广西宜州,546300 |
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| 基金项目： | 广西教育厅项目(201010LX457) |
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| 摘 要： | 目的:探讨超声波协同复合酶法对半边莲多糖提取率的影响.方法:以半边莲为原料,用超声波协同复合酶法提取多糖,通过单因素和正交试验对半边莲多糖的提取工艺进行优化.结果:超声波协同复合酶法提取半边莲多糖的最佳工艺条件为pH4.0,料液比1∶40,超声波时间25 min,复合酶用量1.0％.结论:超声波协同复合酶法具有提取率高、工艺简单、节能的特点.
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| 关 键 词： | 半边莲 多糖 超声波 复合酶 |
| 收稿时间： | 2011/10/3 0:00:00 |
Optimization of Extraction Technology for Polysaccharide from Lobelia chinensis by Ultrasonic-Composite Enzyme Synergistic Method |
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| HUANG Xiu-xiang,LAI Hong-fang and LUO Li-cheng.Optimization of Extraction Technology for Polysaccharide from Lobelia chinensis by Ultrasonic-Composite Enzyme Synergistic Method[J].China Journal of Experimental Traditional Medical Formulae,2012,18(5):44-46. |
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| Authors: | HUANG Xiu-xiang LAI Hong-fang and LUO Li-cheng |
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| Institution: | Department of Chemistry and Life Sciences, Hechi University, Yizhou 546300, China;Department of Chemistry and Life Sciences, Hechi University, Yizhou 546300, China;Department of Chemistry and Life Sciences, Hechi University, Yizhou 546300, China |
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| Abstract: | Objective:To investigate effect of ultrasonic-composite enzyme synergistic method on extraction rate of polysaccharide from Lobelia chinensis.Method:With L.chinensis as raw material,polysaccharide was extracted by ultrasonic-composite enzyme synergistic method,extraction technology of polysaccharide from L.chinensis was optimized by single factor test and orthogonal test.Result:Optimum technology conditions were as follows: pH 4.0,ratio of solid-liquid 1∶40,ultrasonic time 25 min,the amount of enzyme 1.0%.Conclusion:Ultrasonic-composite enzyme synergistic method had features of high extraction ratio,simple process,energy-saving. |
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| Keywords: | Lobeliae chinensis polysaccharide ultrasonic wave enzyme |
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