Detection of both hepatitis B e antigen and antibody in a single assay using monoclonal reagents |
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Authors: | R B Ferns R S Tedder |
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Institution: | Virology Section, Joint Department of Medical Microbiology, Middlesex Hospital Medical School and University College, School of Pathology, Riding House Street, London W1P7LD, U.K. |
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Abstract: | Monoclonal antibodies raised against HBeAg were used to develop a HBeAg and anti-HBe detection assay. Monoclones containing anti-HBe were used both for the coating of the solid phase and for the fluid phase label in a sandwich type assay. The percentage binding of 125I-labelled anti-HBe to serum HBeAg was much greater than that seen in a similar assay using only polyclonal reagents. Therefore it was possible to add a small quantity of HBeAg for neutralising any anti-HBe present in a test serum without affecting HBeAg detection. This small amount of serum HBeAg was incorporated into each test sample thus allowing the determination of the e status of a patient using only one aliquot of test serum. This single test assay could be performed either as a radioimmunoassay or as an ELISA. The sensitivity of these assays was found to be greater than the conventional polyclonal assay particularly with regard to sera containing anti-HBe. |
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Keywords: | hepatitis B e antigen monoclonal antibody single test assay RIA ELISA |
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