Efficient transformation of rat 3Y1 cells by human adenovirus type 9 |
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| Authors: | J S Brusca R Jannun G Chinnadurai |
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| Affiliation: | Institute for Molecular virology, St Louis University Medical Center, St Louis, Missouri 63110, USA |
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| Abstract: | Infection with herpes simplex viruses (HSV) lead to a significant increase of the simian virus 40 (SV40) DNA content in the SV40-transformed hamster cell lines CO631 and Elona. Analysis of this gene-amplifying activity revealed (i) that it cosedimented with infectious herpesvirions in sucrose density gradients, (ii) that it was abolished by anti-HSV antibodies or (iii) by antiviral drugs acting on the HSV-induced DNA polymerase; and analysis of temperature-sensitive mutants showed that this DNA polymerase was an essential component of HSV-induced, gene-amplifying activity in SV40-transformed hamster cells. |
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| Keywords: | Author to whom requests for reprints should be addressed. |
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