| 高效液相色谱法测定八珍颗粒中芍药苷含量 |
| |
| 引用本文： | 谢静,雷灼雨,蒋万浪. 高效液相色谱法测定八珍颗粒中芍药苷含量[J]. 中国药业, 2008, 17(13): 26-27 |
| |
| 作者姓名： | 谢静 雷灼雨 蒋万浪 |
| |
| 作者单位： | 重庆市药品检验所,重庆,401121 |
| |
| 摘 要： | 目的建立八珍颗粒中芍药萝含量测定的高效液相色谱（HPLC）法。方法用50％甲醇提取样品，采用C18色谱柱，以乙腈-0．1％磷酸溶液-三乙胺（13：87：0.04）为流动相，检测波长为230nm，流速为1mL／min。结果芍药苷进样量在0．06018～0．66198μg范围内与峰面积呈线性关系，有糖型和无糖型样品的平均回收率为97．24％和99．55％，RSD为0．53％和0．80％（n=6）。结论HPLC法快速、简便、重现性好，可用于八珍颗粒的质量控制。
|
| 关 键 词： | 八珍颗粒 芍药苷 高效液相色谱法 |
Determination of Peoniflorin in Bazhen Granules by HPLC |
| |
| Xie Jing,Lei Zhuoyu,Jiang Wanlang. Determination of Peoniflorin in Bazhen Granules by HPLC[J]. China Pharmaceuticals, 2008, 17(13): 26-27 |
| |
| Authors: | Xie Jing Lei Zhuoyu Jiang Wanlang |
| |
| Affiliation: | (Chongqing Institute for Drug Control, Chongqing, China 401121) |
| |
| Abstract: | Objective To establish a HPLC method for the determination of peoniflorin in Bazhen Granules. Methods 50% methanol was used as the solvent to prepare the samples. C18 was used as the stationary phase. Acetonitrile-10% phosphoric acid-triethylamine （13：87：0. 04） was used as the fluid phase. The detection wavelength was 230 nm. The flow rate was 1.0 mL/min. Results The linear range of peoniflorin was 0. 060 18 - 0. 661 98 μg. The average recovery rate were 97.24% and 99.55% （ n = 6） with the RSD were 0.53% and 0. 80%. Conclusion The methods is simple,accurate,reproducible and suitable for the quality control. |
| |
| Keywords: | Bazhen Granules peoniflorin HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
