直肠癌p53、K-ras基因突变与临床病理的相关性研究

目的　探索p5 3、k ras基因同时突变对直肠癌的恶性行为升级的作用及其临床意义。方法　用PCR SSCP方法检测直肠癌细胞p5 3、K ras基因突变 ,分析该基因突变与临床病理因素及预后的关系。结果　直肠癌细胞p5 3、K ras基因突变与临床病理因素无关 ,生存率也无统计学差异。结论　直肠癌细胞p5 3、K ras基因突变对癌细胞恶性生物学行为升级无明显的促进作用 ,p5 3、K ras基因同时突变也无协同促癌作用 ,也不影响病人的预后。

Study on the relationship between p53, K-ras gene mutations andtheir clinicopathologic significance in rectal cancer

Objective To explore whether simultaneous occurrence of p53 and K-ras gene mutation could promote the grades of malignant biologic behavior of specimens, and its clinicopathologic significance in rectal cancer.Methods p53 and K-ras gene mutations were detected by PCR-SSCP, and the relationships between the gene mutations and clinicopathologic, prognostic factors were also studied.Results There was no association between clinicopathologic factors and the p53 and K-ras gene mutation. No statistical difference was found between the survival rate and mutations.Conclusion The grades of malignancy of rectal cancer are not influenced by the gene mutations and the simultaneous occurrence of p53 and K-ras gene mutation has no synergic effects on the promotion of rectal cancer and the prognosis.