| 薯蓣皂苷元对大鼠成骨细胞增殖、分化及OPG/RANKL mRNA表达的影响 |
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| 引用本文： | 江洋珍,王俊俊,梁继超,陈勇. 薯蓣皂苷元对大鼠成骨细胞增殖、分化及OPG/RANKL mRNA表达的影响[J]. 中华中医药杂志, 2010, 0(1) |
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| 作者姓名： | 江洋珍 王俊俊 梁继超 陈勇 |
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| 作者单位： | 湖北大学中药生物技术省重点实验室; |
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| 摘 要： | 目的:研究薯蓣皂苷元对体外培养的大鼠成骨细胞增殖、分化及护骨素/核因子κB受体活化因子配基(OPG/RANKL) mRNA的影响,探讨薯蓣皂苷元预防与治疗骨质疏松的作用机制。方法:在体外培养的大鼠成骨细胞培养基中分别加入不同浓度的薯蓣皂苷元作用不同时间,用MTT法检测药物对成骨细胞增殖的影响,检测成骨细胞内ALP活性分析药物对成骨细胞分化的影响,用半定量RT-PCR检测成骨细胞内OPG/RANKL mRNA的表达量,分析药物对破骨细胞重要信号传导通路OPG/RANKL/RANK系统的影响。结果:3.64×10-8-3.64×10-7mol/L的薯蓣皂苷元作用于大鼠成骨细胞3d,可促进成骨细胞的增殖(P<0.01),上调成骨细胞内OPG/RANKL mRNA的比值(P<0.01);作用7d,可提高成骨细胞内ALP活性(P<0.05或P<0.01)。结论:适量浓度薯蓣皂苷元可促进成骨细胞的增殖、分化及抑制破骨细胞的形成。
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| 关 键 词： | 薯蓣皂苷元 成骨细胞 增殖 分化 mRNA表达 |
Effects of diosgenin on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblasts cultured in vitro |
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| JIANG Yang-zhen,WANG Jun-jun,LIANG Ji-chao,CHEN Yong. Effects of diosgenin on cell proliferation,differentiation and OPG/RANKL mRNA expression of rat osteoblasts cultured in vitro[J]. China Journal of Traditional Chinese Medicine and Pharmacy, 2010, 0(1) |
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| Authors: | JIANG Yang-zhen WANG Jun-jun LIANG Ji-chao CHEN Yong |
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| Affiliation: | Hubei Province Key Laboratory of Biotechnology of Chinese Materia Medica;Hubei University;Wuhan 430062;China |
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| Abstract: | Objective:To study the effects of diosgenin on cell proliferation, differentiation and OPG/RANKL mRNA expression of rat osteoblasts cultured in vitro for analyzing the prevention and treatment mechanism of diosgenin. Methods: Rat osteoblast cultured in vitro was treated by a series of different concentrations of diosgenin for different time respectively. The proliferation of osteoblast was detected by MTT method. The differentiation of osteoblast was detected by the activity of ALP mRNA expression of osteop... |
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| Keywords: | Diosgenin Osteoblast Proliferation Differentiation mRNA expression |
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