| 右心室肥大时心肌间质胶原改建及其前胶原基因的表达 |
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| 引用本文： | 万新红,孙明. 右心室肥大时心肌间质胶原改建及其前胶原基因的表达[J]. 中华心血管病杂志, 1999, 27(3): 207-209 |
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| 作者姓名： | 万新红 孙明 |
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| 作者单位： | 汕头大学医学院第二附属医院心内科(万新红)，湖南医科大学附属湘雅医院(孙明)，湖南医科大学第二附属医院(谢景超)，湖南医科大学肿瘤研究所(曹亚) |
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| 摘 要： | 目的了解法乐四联症肥大右室心肌间质Ⅰ、Ⅲ型胶原的改建及与前胶原基因ｍＲＮＡ表达的关系，为防治心肌间质胶原改建提供新的依据和途径。方法用生化方法定量分析了２２例法乐四联症患者和８例非心血管及胶原系统疾病尸检者的右室心肌间质中的胶原含量和Ⅰ、Ⅲ型胶原比值，以及用斑点杂交法测定法乐四联症患者右室心肌间质Ⅰ、Ⅲ型前胶原基因ｍＲＮＡ的表达。结果法乐四联症组心肌间质胶原含量为（１４８５±２４８）μｇ／ｍｇ总蛋白，较对照组（７５５±１８９）μｇ／ｍｇ总蛋白明显增多（Ｐ＜００５）；其Ⅰ／Ⅲ型胶原比值为５１０±１８４，较对照组２２７±０５８显著增高（Ｐ＜００５）；法乐四联症组右室心肌间质胶原含量与Ⅰ、Ⅲ型前胶原基因表达强度的相关系数分别为０８０（Ｐ＜０００１）、－０２７７（Ｐ＞０１０）。右室心肌间质胶原Ⅰ／Ⅲ型比值与Ⅰ／Ⅲ型前胶原基因表达强度比值呈正相关，ｒ＝０４７（Ｐ＜００５）。结论提示法乐四联症肥大右室心肌间质存在以胶原含量增多和Ⅰ／Ⅲ型比值增高为特点的胶原改建，且主要与Ⅰ型前胶原基因ｍＲＮＡ转录水平上调有关。
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| 关 键 词： | 法乐氏四联症 心肌 胶原 纤维变性 |
Remodeling of myocardial interstitial collagen and procollagen gene expression |
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| WAN Xinhong,SUN Ming,XIE Jingchao,et al The Second Affiliated Hospital,Medical College of Shantou University,Shantou. Remodeling of myocardial interstitial collagen and procollagen gene expression[J]. Chinese Journal of Cardiology, 1999, 27(3): 207-209 |
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| Authors: | WAN Xinhong SUN Ming XIE Jingchao et al The Second Affiliated Hospital Medical College of Shantou University Shantou |
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| Affiliation: | WAN Xinhong*,SUN Ming,XIE Jingchao,et al * The Second Affiliated Hospital,Medical College of Shantou University,Shantou 515041 |
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| Abstract: | Objective To investigate the characteristics and procollagen gene expression of myocardial interstitial collagen of right ventricle in tetralogy of Fallot (TOF) Methods Samples of the infundibular septum from 22 patients with TOF and 8 patients without cardiovascular disease and collagen system disease as control were studied Collagen was determined by biochemical methods, Pro (I) mRNA, Pro (III) mRNA levels were determined by dot blot hybridization Results The hydroxyproline concentration and the ratio of type / collagen in the right ventricle with TOF were significantly higher than those in controls s) g/mg total protein vs 755189 g/mg total protein, respectively, P<005; 510184 vs 227058, P<005, respectively] The correlation between the hydroxyproline concentration with the level of Pro ()mRNA, Pro () mRNA were 080 (P<0001) and -0277 (P>010), respectively The correlation between the ratio of type I/III collagen and the ratio of type / procollagen mRNA was positive (r=047, P<005) Conclusion Increased collagen and the ratio of type / collagen are the characteristics in collagen remodeling of the hypertrophic right ventricle in TOF Enhanced type procollagen gene expression may be the cause of the increased collagen synthesis |
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| Keywords: | tetralogy of Fallotmyocardiumcollagenfibrosis |
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